Enzyme For General Recombination

Question 1

Function of RecBCD

Answer 1

Initiate recombination

Question 2

Function of RecA

Answer 2

Bind to ssDNA ->nucleoprotein filament

=> strand invasion and homologous pairing

Question 3

Function of RuvA,B,C?

Answer 3

Drive branch migration;

Process Holliday junction into recombinant pdts

Question 4

Describe the process of RecBCD

Answer 4

Initiate recombination: attaching to the end of a DNA duplex; unwind dsDNA via helicase and nuclease activity

SSB binds to the ss;

RecBCD endonuclease cleaves ssDNA;

RecBCD directs the binding of RecA to 3’-terminal strand;

Nucleoprotein filament formed-> nucleoprotein filament capable of homologous pairing with a dsDNA and strand invasion

Question 5

RecA aka?

Answer 5

Recombinase

Question 6

RecA is capable of?

Answer 6

Mediates ATP dependent DNA strand exchange reaction

-> formation of Holliday junction