enzyme analysis Flashcards
1
Q
what are we doing in this practical?
A
using iodine to test if starch is present
2
Q
colour it will turn if it is?
A
blue/black
3
Q
step 1?
A
using a spotting tile, place one drop of iodine in each
4
Q
get three test tubes of 1.? 2.? 3.? (step 2 )
A
- starch 2. amylase solution 3. pH 5 buffer (all 2cm^3)
5
Q
what do you do with these tubes? (step 3 )
A
place all in water bath at 30 degrees for 10 minutes
6
Q
after leaving them? (step 4 )
A
combine all solutions
7
Q
after combining? (step 5 )
A
return to water bath
8
Q
after returning how long do you wait and what do you do? (step 6 )
A
30 seconds, put one drop into a dimple
9
Q
what will it do? (step 7 )
A
turn blue black
10
Q
continue until? (final step)
A
iodine turns orange when added, this means reaction is complete and starch is no longer present. then repeat using different pH buffers.
