Enzyme Activity Flashcards
enzyme concentration
increased number of active sites
more enzyme-substrate form until substrate conc becomes limiting factor
Substrate Concentration
increased number of enzyme-substrate complex
until all enzymes are working still leaving substrate to be brocken down
enzyme conc will become limiting factor
pH
each enzyme has optimum pH. Above or below decreases rate of reaction
affects hydrogen bonds that holds the active site
Temperature
have optimum temp
increase = increase in kinetic energy and rate of reaction
above optimum rate rate of reaction decreases until enzyme denatures
Competitive inhibitor
- compete for the active site of enzyme
active site blocked not able to catalyse the reaction. reaction slows down
non competitive inhibitor
binds to the allosteric site
causing the enzyme’s active site to change
rate of reaction decreases drastically
lock + key model
enzyme active site is complementary to substrate
fits on collision
coplex formed
reaction occurs
induced fit model
the active site is not completely complimentary to the substrate
a collision occurs, the enzyme changes its shape slightly to fit the active site around the substrate
complex formed
reaction occurs