Enzyme Action Flashcards

Thursday 12th September 2019

You may prefer our related Brainscape-certified flashcards:
1
Q

What does denatured mean? What causes it?

A

When the shape of an enzyme is changed so that its substrate no longer fits in the active site, so it can’t be broken down into its products.
Caused by - temperature over 37C, pH over

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2
Q

What is a model?

A

A visual representation of an idea - normally simple. An example - he lock & key model

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3
Q

What causes an enzyme to denature?

A

Temperature (over 37C) or pH

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4
Q

What do catalysts such as enzymes do?

A

Reduce the activation energy needed for a reaction. This means more collisions will be successful and end in a reaction

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5
Q

The substrate is
a) complementary
b) complimentary
to an enzyme’s active site (lock and key)

A

a) complEmentary

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6
Q

Why doesn’t the lock & key model work? (1894)

A

What stops the substrate from falling out of the enzyme-substrate complex?

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7
Q

Talk about the Induced Fit hypothesis (1958. Daniel Koshland)

A

The substrate isn’t 100% complementary first. It goes into the enzyme’s active site, and the enzyme moulds slightly around it
Really weak - hydrogen bonds, ionic attractions, van de Waals forces & hydrophobic interactions - non covelant forces. These bond the substrate to the active site.
When the product is formed, slightly different shape to the substrate, so it detaches from the active site
Enzyme + substrate –> Enzyme-substrate –> Enzyme-product complex –> enzyme + products

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8
Q

What are the bonds between an enzyme and the substrate in induced fit hypothesis?

A

really weak - hydrogen bonds, ionic attractions, van de Waals forces & hydrophobic interactions - non covelant forces

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9
Q

Give the word equation for

a) Lock & key hypothesis
b) Induced fit hypothesis

A

a) Enzyme + substrate –> enzyme-substrate complex –> enzyme + products
b) Enzyme + substrate –> Enzyme-substrate –> Enzyme-product complex –> enzyme + products

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10
Q

What is the difference between an enzyme-substrate complex & an enzyme-product complex?

A

An enzyme-substrate complex has no bonds connecting the 2 at all (lock & key), as it fits perfectly/ are complementary. An enzyme-product complex used to have bonds fitting the 2, but now the products are a different shape to the enzyme’s active site, so there are no bonds between the 2

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11
Q

If the active site can mould to fit a substrate, why does the body need different types of enzymes - wouldn’t the same one work for all substrates?

A

The enzyme can only change a little bit. However, some enzymes can break down more than one substrate if they have similar shapes

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12
Q

When asked to find a rate of reaction on a graph, what do you do if asked:

a) What’s the ROR at 20 seconds?
b) What’s the ROR across the first 20 seconds?

A

a) Draw a tangent. One Point, One Line!

b) Gradient

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13
Q

When proteins are heated to a high temperature, their tertiary structure is disrupted. Explain how this occurs (3 marks)

A

1) Weak hydrogen bonds are broken (made in tertiary structrue)
2) Protein unfolds
3) Change in the 3D shape, so the enzyme has denatured

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14
Q

At what temperature is the optimum for enzymes?

A

37C (same as internal temperature of humans. Coincidence? no, definitely not)>

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15
Q

Why does increasing the temperature increase the rate of a enzyme based reaction?

A

The substrate & enzyme molecules have more kinetic energy. So, they can move more and faster, leading to more frequent collisions, so more are successful.
More enzyme-substrate complexes, so faster ROR

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16
Q

Why does pH denature an enzyme?

A

Ionic bonds break very easily with slight pH changes. Ionic bonds are formed in tertiary structure, so when broken, it causes the protein to unfold. Loses its 3D shape - denatured

17
Q

How does increasing the substrate concentration lead to a higher ROR?

A

More substrate, so more enzymes can work at the same time. So, more enzyme-substrate complexes can form, more product formed - increasing ROR.
Substrate concentration was the limiting factor

18
Q

When a graph plateaus, what does this often mean?

A

The x axis is no longer the limiting factor

19
Q

In a lab, the substrate concentration of an enzyme concentration can easily be changed. How does it happen in living cells?

A

The enzyme concentration is controlled by the cell - if it needs more enzymes, it makes more. But, it can’t control the substrate concentration.
Reactions & making enzymes take energy, so enzymes only made when the product is NEEDED

20
Q

If you kept increasing enzyme and substrate concentration for infinity, what would theoretically happen?

A

The reaction would continue endlessly until something else (temperature denaturing enzymes, pH) is the limiting factor