ENZYME

Question 1

It is a non protein entities

Answer 1

Cofactors

Question 2

Example of a cofactor

Answer 2

Inorganic substances like non metals and minerals

Question 3

Release after catalysis is called

Answer 3

Coenzymes

Question 4

Alters spatial configuration of the enzyme for proper substrate binding

Answer 4

Activators

Question 5

Example of coenzyme

Answer 5

Vitamins

Question 6

Example of Activators

Answer 6

Calcium Ion

Question 7

Interferes with the enzymatic reactions

Answer 7

Inhibitors

Question 8

Binds to the active site of an enzyme

Answer 8

Competitive inhibitor

Question 9

Binds to the allosteric site (Cofactor site)

Answer 9

Noncompetitive inhibitor

Question 10

Binds to the enzyme-substrate complex

Answer 10

Uncompetitive inhibitor

Question 11

Without _______ protein will not be activated and enzyme cannot function without factors.

Answer 11

Cofactors

Question 12

Optimum temperature of enzyme activity

Answer 12

37 degree Celsius

Question 13

Denaturation of enzymes

Answer 13

40-50 degrees Celsius

Question 14

Inactivation of enzymes

Answer 14

60-65 degrees Celsius

Question 15

For every 10 C increase in temperature, there will be a two-fold increase in enzyme activity.

Answer 15

Temperature coefficient (Q10)

Question 16

At what range does most physiologic reactions occur in pH?

Answer 16

7-8

Question 17

Storage for enzyme

Answer 17

-20 C

Question 18

Storage for substrate and coenzymes

Answer 18

2-8 C

Question 19

Storage for LD 4 and 5

Answer 19

Room Temperature

Question 20

Increases enzyme concentration

Answer 20

Hemolysis

Question 21

Decreases enzyme concentration

Answer 21

Latescence or milky specimen

Question 22

Enzyme nomenclature

Answer 22

1st digit: Classification
2nd and 3rd digits: subclass
4th digits: serial number

Question 23

Enzyme classification

Answer 23

a. Oxidoreductases
b. Transferases
c. Hydrolases
d. Lyases
e. Isomerases
f. Ligases

Question 24

Transfer of a chemical group other than hydrogen from 1 substrate to another

Answer 24

Transferases

Question 25

splitting by addition of water - Hydrolysis

Answer 25

Hydrolases

Question 26

removal of groups without hydrolysis

Answer 26

Lyases

Question 27

Redox reduction

Answer 27

Oxidoreductases

Question 28

Intramolecular arrangements

Answer 28

Isomerases

Question 29

Joining of 2 substrate molecules synthases

Answer 29

Ligases

Question 30

Where the substrate interacts

Answer 30

Active site

Question 31

May bind regulatory molecules

Answer 31

Allosteric site

Question 32

coenzyme that bound tightly to the enzyme

Answer 32

Prosthetic group

Question 33

an enzyme along with its cofactor is called

Answer 33

Haloenzyme

Question 34

Inactive form of enzyme

Answer 34

zymogen/proenzyme

Question 35

shape of the key must fit into the lock

Answer 35

Lock and key theory

Question 36

Based on the substrate binding to the active site of the enzyme

Answer 36

Induced fit theory

Question 37

Enzyme combines with only 1 substrate and catalyzes only 1 reaction

Answer 37

Absolutely specificity

Question 38

Enzyme combines with all the substrates in a chemical group.

Answer 38

Group specificity

Question 39

Enzymes reacting with specific chemical bonds

Answer 39

Bond specificity

Question 40

Reaction rate depends only on enzyme concentration

Answer 40

Zero-order reaction

Question 41

Reaction rate is directly proportional to substrate concentration

Answer 41

First-order reaction

Question 42

International unit for enzyme

Answer 42

1 micromole of substrate /minute

Question 43

Katal unit

Answer 43

1 mole of substrate/second

Question 44

Absorbance is made at 10-second intervals for 100 seconds

Answer 44

Nonkinetic assay

Question 45

Electrophoresis:

Answer 45

(+) Liver - Bone (Regan) - Placenta- Intestine (-)

Question 46

Heat fractionation:

Answer 46

(Stable) Regan - Placenta - Intestine - Liver- Bone (Labile)

Question 47

Inhibits Regan, placental and intestinal ALP

Answer 47

Phenylalanine

Question 48

Inhibits Nago ALP

Answer 48

L-Leucine

Question 49

Inhibits live and bone ALP

Answer 49

Levamisole

Question 50

Inhibits bone ALP

Answer 50

3M urea