enzymatic reactions definition and control Flashcards
(22 cards)
biochemistry =
study of life processes
three-layered approach
-chemical structures of molecules
-interactions between molecules
-metabolism: how all the structures/interactions fit together
most chemical reactions are reversible under…
physiological conditions
what do forward and backward reactions create ?
kinetic equilibrium with equal generation of both substrate and product
what is kinetic steady-state ?
where metabolites flow through pathways (this is what cells are considered as)
is metabolism fixed or dynamic ?
dynamic, it responds to cellular and organism changes
what does metabolism involve ?
the linked reactions in a cell
(no reaction occurs in isolation)
what are substrates and products referred to ?
metabolites
what are substrates and products referred to ?
metabolites
three main ways to represent metabolic pathways:
-linear
-branched
-cyclical
how do cell compartments aid in reactions ?
helps to concentrate the enzymes and metabolites to accelerate the reaction
what do multi-enzyme complexes allow ?
direct transfer of metabolites
atomic/molecular rearrangement reactions
changing the position of atoms or functional groups WITHIN a molecule (not replacing it)
substitution reactions
replacement of one group of atoms with another
transfer reactions
transfer of a functional group to substrate
redox reactions
dual oxidation and reduction reactions that usually involve co-enzymes
cleavage reactions
hydrolysis and splitting molecules in two
condensation reactions
two molecules are joined together with the loss of water
addition reactions
-two molecules are joined together but water is NOT eliminated
-addition across a double bond
what influences rate of enzymatic reactions ?
-concentrations of:
=enzyme
=substrate
=activators
=inhibitors
=coenzymes
the relationship between substrate concentration and reaction rate
-zone 1: first order kinetics with regard to
substrate
-zone 2: transitional zone
-zone 3: increasing substrate conc. has no
effect