enzy matic methods Flashcards
Subject to many interference
A. Polagraphic
B. Peroxidase-coupled Trinder reaction
B. Peroxidase-coupled Trinder reaction
measurement of the degree of Oz consumption using a p O2 Clark electrode
A. Polagraphic
B. Peroxidase-coupled Trinder reaction
A. Polagraphic
H202 + chromogen– ** peroxidase**-> oxidized chromogen + H20
a) trinder
b) polarographic
a
color of oxidized chromogen
A. quinoneimine red/red purple
B. quinone mine . red/red blue
A. quinoneimine red/red purple
Requires addition of molybdate and iodide or catalase
and ethanol
a. Trinder / peroxidase
b. polarographic
b. polarographic
MOST SPECIFIC enzyme reacting with only β-D-Glucose
A.. Glucose oxidase
B. Hexokinase
C. Glucose dehydration
A.. Glucose oxidase
UV method / Reference method for glucose determination
A. Hexokinase
B. Glucose oxidase Method
A. Hexokinase
Uses: Hexokinase & G-6-PD
A. Hexokinase
B Glucose oxidase messia
A. Hexokinase
highly specific for glucose, not subject to interference
A. Hexokinase
B. Glucose Dehydrogenase
A. Hexokinase