enzy matic methods Flashcards

1
Q

Subject to many interference
A. Polagraphic
B. Peroxidase-coupled Trinder reaction

A

B. Peroxidase-coupled Trinder reaction

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2
Q

measurement of the degree of Oz consumption using a p O2 Clark electrode

A. Polagraphic
B. Peroxidase-coupled Trinder reaction

A

A. Polagraphic

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3
Q

H202 + chromogen– ** peroxidase**-> oxidized chromogen + H20
a) trinder
b) polarographic

A

a

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4
Q

color of oxidized chromogen
A. quinoneimine red/red purple
B. quinone mine . red/red blue

A

A. quinoneimine red/red purple

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5
Q

Requires addition of molybdate and iodide or catalase
and ethanol

a. Trinder / peroxidase
b. polarographic

A

b. polarographic

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6
Q

MOST SPECIFIC enzyme reacting with only β-D-Glucose

A.. Glucose oxidase
B. Hexokinase
C. Glucose dehydration

A

A.. Glucose oxidase

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7
Q

UV method / Reference method for glucose determination

A. Hexokinase
B. Glucose oxidase Method

A

A. Hexokinase

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8
Q

Uses: Hexokinase & G-6-PD
A. Hexokinase
B Glucose oxidase messia

A

A. Hexokinase

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9
Q

highly specific for glucose, not subject to interference

A. Hexokinase
B. Glucose Dehydrogenase

A

A. Hexokinase

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