ENTEROBACTERIACEAE- Salmonella And Shigella

Question 1

What are the distinguishing feature of ENTEROBACTERIACEAE?

Answer 1

Gram (-), Oxidase (-), Catalase (+), Cytochrome c oxidase (-)
Facultative anaerobes, Ferment glucose, Reduce nitrates to nitrites.
Endotoxin and some of it are exotoxin, Antigenes ( O, H, K, Vi).

Question 2

What are the distinguishing feature of S.typhi?

Answer 2

Gram(-), oxidase(-), lactose(-), H2S(+), urease(-).

Facultative anaerobe, sensetive to acid, highly motile, no animal resorvoir.

Question 3

Pathogenesis and diseases of S.typhi?

Answer 3

Typhid and paratyphoid fever.
Iliocecal (constipation) — host cell ruffle — M cell(no mucous) phagocyte it — pyres patches — mesenteric lymph nodes — blood.

Question 4

Pathogenesis of S.typhi?

Answer 4

Blood — septicemia(fever)
Can cause rose spots on trunk and abdomen.
Replicate in macrophages.

Question 5

Complication of S.typhi?

Answer 5

Necrosis of peyer patches with perforation, thrombophlebitis, cholycestitis, pneumonia, abcsess formation.

Question 6

How the S.typhi can invades the cell?

Answer 6

M cell adhesion and translocation
Direct invation of epithelia cells
Luminal capture by dendritic cells

Question 7

Diagnosis of S.typhi?

Answer 7

Isolated from blood, urine, bone marrow, biopsy from rose spots, agglutination detected the antibodies to antigenes.

Question 8

Prevention of S.typhi?

Answer 8

Sanitation and we have 3 vaccine (strain 21, parenteral heat killed typhi, parenteral ViCPS).

Question 9

What are the distinguishing feature of S.enteritidis and S.typhimurium?

Answer 9

Gram (-), motile, non lactose fermenting, produce H2S, exsist in enteric tract of human and domestic animals.

Question 10

Pathogenesis of this subspescies?

Answer 10

Sensetive to acid, endotoxin, no exo, shallow ulceration, invades iliocecal region— loose diarrhea.

Question 11

What is the first and second most common bacterial cause of Enterocolitis/gastroenteritis?

Answer 11

First — campylobacter

Second — Salamonella

Question 12

What is the most common cause of osteomyelitis in sickle cell disease?

Answer 12

Salmonella

Question 13

What are diseases of salmonella subspecies?

Answer 13

Enterocolitis/gastroenteritis, septicemia, osteomylitis

Question 14

Diagnosis of salmonella subspecsies?

Answer 14

Culture on hekton agar and H2S production

Question 15

What are the distinguishinf feature of shigella?

Answer 15

Gram (-), non lactose ferment, non motile, exsist in colon, acid resistance, ampicillin resistance.

Question 16

Pathogenesis of shigella?

Answer 16

Endotoxin, invades M cells, no H antigen, produce shallow ulcer, toxin( type 1 produce by desenteriae, neurotoxin, cytotoxin, nterotoxin, AB component)

Question 17

Diseases of shigella?

Answer 17

Enterocollitis, shigellosis — dysentry
Water diarrhea — bloody diarrhea
Rectal prolapse

Question 18

Diagnosis of shigella?

Answer 18

Culture of stool