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Enterobacteriaceae Flashcards

1
Q

What family do Enterobacteriaceae belong to?

A

They belong to the family Enterobacteriaceae.

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2
Q

True or False: Enterobacteriaceae are primarily gram-positive bacteria.

A

False

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3
Q

Name one common genus within the Enterobacteriaceae family.

A

Escherichia

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4
Q

Fill in the blank: Enterobacteriaceae are typically _______ shaped.

A

rod

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5
Q

What is the primary habitat of Enterobacteriaceae?

A

The intestinal tract of humans and animals.

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6
Q

Which species of Enterobacteriaceae is commonly associated with urinary tract infections?

A

Escherichia coli

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7
Q

True or False: All Enterobacteriaceae are pathogenic.

A

False

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8
Q

What type of metabolism do Enterobacteriaceae primarily exhibit?

A

Facultative anaerobic metabolism.

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9
Q

Name a common disease caused by pathogen members of Enterobacteriaceae.

A

Gastroenteritis

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10
Q

Multiple Choice: Which of the following is NOT a member of Enterobacteriaceae? A) Salmonella B) Shigella C) Streptococcus D) Klebsiella

A

C) Streptococcus

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11
Q

What is the significance of the lactose fermentation test in identifying Enterobacteriaceae?

A

It helps differentiate lactose fermenters from non-fermenters.

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12
Q

Fill in the blank: Enterobacteriaceae can be identified using _____ tests.

A

biochemical

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13
Q

True or False: Enterobacteriaceae are resistant to all antibiotics.

A

False

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14
Q

What is a common method for isolating Enterobacteriaceae from clinical samples?

A

Culture on selective media.

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15
Q

Which Enterobacteriaceae is known for causing foodborne illness and is often found in undercooked meat?

A

Salmonella enterica

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16
Q

What diagnostic test can be used to confirm the presence of E. coli O157:H7?

A

Serotyping

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17
Q

True or False: Enterobacteriaceae can be found in soil and water environments.

A

True

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18
Q

Which virulence factor is commonly associated with pathogenic strains of Enterobacteriaceae?

A

Endotoxin (lipopolysaccharide)

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19
Q

Fill in the blank: Enterobacteriaceae can be differentiated from other bacteria by their ability to ferment _______.

A

glucose

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20
Q

Name a test used to identify the urease activity of Enterobacteriaceae.

A

Urease test

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21
Q

Multiple Choice: Which of the following is a non-lactose fermenting Enterobacteriaceae? A) E. coli B) Enterobacter C) Salmonella D) Klebsiella

A

C) Salmonella

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22
Q

What type of infections can Klebsiella pneumoniae cause?

A

Pneumonia and bloodstream infections.

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23
Q

Fill in the blank: Enterobacteriaceae are often treated with _______ antibiotics.

A

beta-lactam

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24
Q

True or False: Enterobacteriaceae are known to have plasmids that confer antibiotic resistance.

A

True

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25
What role do Enterobacteriaceae play in the human microbiome?
They contribute to digestion and nutrient absorption.
26
Name a common laboratory medium used for the growth of Enterobacteriaceae.
MacConkey agar
27
28
What is the primary biochemical test used to identify Escherichia coli?
Indole test
29
True or False: Escherichia coli is a Gram-negative bacterium.
True
30
Fill in the blank: Escherichia coli ferments ______ to produce gas.
lactose
31
Which biochemical test differentiates E. coli from Enterobacter species?
Indole test
32
What color does E. coli turn on MacConkey agar?
Pink
33
True or False: E. coli is oxidase positive.
False
34
What does a positive methyl red test indicate about E. coli?
Mixed acid fermentation
35
Which test indicates the ability of E. coli to utilize citrate?
Citrate test
36
What is the expected result of the urease test for E. coli?
Negative
37
E. coli is typically _______ for hydrogen sulfide production.
Negative
38
What is the result of the Voges-Proskauer test for E. coli?
Negative
39
What type of fermentation does E. coli primarily perform?
Anaerobic fermentation
40
True or False: E. coli can reduce nitrate to nitrite.
True
41
What biochemical test is used to check for lactose fermentation?
Lactose fermentation test
42
Which enzyme is detected in the catalase test for E. coli?
Catalase
43
What is the result of the citrate test for E. coli?
Negative
44
Fill in the blank: E. coli is _______ for the urease test.
Negative
45
What does a positive indole test indicate?
Presence of tryptophanase
46
Which test is used to identify E. coli's ability to ferment glucose?
Glucose fermentation test
47
True or False: E. coli produces gas from glucose fermentation.
True
48
What color change indicates a positive result in the methyl red test?
Red
49
What is the purpose of the oxidase test?
To determine the presence of cytochrome c oxidase
50
What is the expected result of the oxidase test for E. coli?
Negative
51
Which test can differentiate E. coli from Klebsiella species?
Indole test
52
What biochemical test indicates the ability of an organism to utilize starch?
Starch hydrolysis test
53
Fill in the blank: E. coli is _______ for hydrogen sulfide production.
Negative
54
What is the significance of a positive lactose fermentation test?
Indicates the presence of coliform bacteria
55
What does a positive citrate test indicate?
Ability to utilize citrate as the sole carbon source
56
True or False: E. coli can produce urease.
False
57
What is the primary fermentation product of E. coli?
Lactic acid
58
Which test is used to determine the ability of E. coli to produce acid from lactose?
Lactose fermentation test
59
What is the result of the methyl red test for E. coli?
Positive
60
What type of bacteria is E. coli classified as based on its oxygen requirements?
Facultative anaerobe

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