ENTEROBACTERIACEAE Flashcards
Enterobacteriaceae (EB) is often referred to as?
Enterics
EB is the normal flora of?
Gastrointestinal tract (Humans and animals)
Morphology of EB
Gram-negative bacilli or coccobacilli
All EB are? (Characteristics)
Facultative anaerobe, glucose fermenter, non-spore forming, cytochrome oxidase negative
All members are motile except
Klebsiella, Shigella, Yersinia
Klebsiella and Shigella are non-motile at what temp
37 deg and RT
Yersinia is non-motile and motile at what temp
NM at 37 deg, M at 25 deg
Motile members have what structure?
Pertrichous flagella
What is its growth on BAP
Moist, smooth, gray, shiny, entire, convex, and opaque
B-hemolysis can be seen in some strains such as
E. coli
Presumptive identification of EB is best done using selective and differential media such as
MacConkey Agar (MAC)
Hektoen Enteric Agar (HEA)
Xylose-lysine deoxycholate Agar (XLD)
Salmonella-Shigella Agar (SSA)
Often part of the usual intestinal microbiota
Opportunistic pathogens
Not present in commensal biota in GI tract of humans
Primary pathogens
Most significant species in the genus of Escherichia
Escherichia coli (Colon Bacillus)
Characteristics of E. coli
Fimbriae, sex pili, motile, and antigens O, H, K
Colony morphology of E. coli
MAC: Lactose fermenter with surrounding area of precipitated bile salts
EMB: Green metallic sheen
Biochemical characteristic of E. coli
Fermenter of: Glucose, lactose, trehalose, xylose
+: Indole, MR
-: VP, Citrate, DNAse, Urease, PAD, H2S
ETEC
Enterotoxic E. coli
Associated with diarrhea of infants and adults (Traveller’s diarrhea)
Enterotoxic E. coli
Serves as a protective mechanism against colonization of ETEC
Stomach acid
Area of colonization of ETEC
Small intestine
Toxins in small intestine released by ETEC
Heat-labile and Heat-stable toxin
EIEC
Enteroinvasive E. coli
MOT of EIEC
Fecal-oral