ENTEROBACTERIACEAE Flashcards

1
Q

Enterobacteriaceae (EB) is often referred to as?

A

Enterics

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2
Q

EB is the normal flora of?

A

Gastrointestinal tract (Humans and animals)

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3
Q

Morphology of EB

A

Gram-negative bacilli or coccobacilli

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4
Q

All EB are? (Characteristics)

A

Facultative anaerobe, glucose fermenter, non-spore forming, cytochrome oxidase negative

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5
Q

All members are motile except

A

Klebsiella, Shigella, Yersinia

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6
Q

Klebsiella and Shigella are non-motile at what temp

A

37 deg and RT

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7
Q

Yersinia is non-motile and motile at what temp

A

NM at 37 deg, M at 25 deg

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8
Q

Motile members have what structure?

A

Pertrichous flagella

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9
Q

What is its growth on BAP

A

Moist, smooth, gray, shiny, entire, convex, and opaque

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10
Q

B-hemolysis can be seen in some strains such as

A

E. coli

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11
Q

Presumptive identification of EB is best done using selective and differential media such as

A

MacConkey Agar (MAC)
Hektoen Enteric Agar (HEA)
Xylose-lysine deoxycholate Agar (XLD)
Salmonella-Shigella Agar (SSA)

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12
Q

Often part of the usual intestinal microbiota

A

Opportunistic pathogens

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13
Q

Not present in commensal biota in GI tract of humans

A

Primary pathogens

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14
Q

Most significant species in the genus of Escherichia

A

Escherichia coli (Colon Bacillus)

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15
Q

Characteristics of E. coli

A

Fimbriae, sex pili, motile, and antigens O, H, K

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16
Q

Colony morphology of E. coli

A

MAC: Lactose fermenter with surrounding area of precipitated bile salts
EMB: Green metallic sheen

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17
Q

Biochemical characteristic of E. coli

A

Fermenter of: Glucose, lactose, trehalose, xylose
+: Indole, MR
-: VP, Citrate, DNAse, Urease, PAD, H2S

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18
Q

ETEC

A

Enterotoxic E. coli

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19
Q

Associated with diarrhea of infants and adults (Traveller’s diarrhea)

A

Enterotoxic E. coli

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20
Q

Serves as a protective mechanism against colonization of ETEC

A

Stomach acid

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21
Q

Area of colonization of ETEC

A

Small intestine

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22
Q

Toxins in small intestine released by ETEC

A

Heat-labile and Heat-stable toxin

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23
Q

EIEC

A

Enteroinvasive E. coli

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24
Q

MOT of EIEC

A

Fecal-oral

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25
Manifestation of EIEC
Bloody diarrhea
26
Virulence test of EIEC
Sereny Test
27
EPEC
Enteropathogenic E. coli
28
STEC
Shiga toxin-producing E. Coli
29
E. coli strains that produce shiga toxin 1 and/or 2
Shiga toxin-producing E. Coli
30
MOT of ETEC
Contaminated food and water
31
Pathophysiology of STEC
Hemolytic Uremic Syndrome
32
EAEC
Enteroaggregative E. coli
33
Aggregative stacked brick patter on the cells and considered a cause of diarrhea in patients with HIV
Enteroaggregative E. coli
34
Widely recognized as the most common cause of UTI
Uropathogenic E. coli
35
Virulence factors of uropathogenic E. coli
Pili - adhere to the urinary epithelial mucosa Cytolysin - kill immune effector cells and inhibits phagocytosis and chemotaxis of certain WBCS Aerobactin - chelates iron Flagella - bacterial dissemination
36
Species that are isolated from urine, blood, wounds, feces, and gallbladder
Escherichia fergusonii
37
Species that are associated with diarrheal disease with some strains carrying stx2f gene
Escherichia albertii
38
Formerly called E.coli atypical or enteric group II
Escherichia hermanii
39
Species that are isolated from humans with infected wounds
Escherichia vulneris
40
It is encapsulated and nonmotile, usually found in the GI tract, free living in soil, water, and plants
Klebsiella spp.
41
Distinct feature of Klebsiella pneumoniae
Polysaccharide capsule
42
Gram-stain of K. pneumoniae
Gram-negative bacilli
43
Klebsiella spp. that resemples K. pneumoniae in its disease spectrum and biochemical properties
Klebsiella oxytoca
44
Klebsiella spp. that may contribute to the disease called ozaena
Klebsiella ozaenae
45
Klebsiella spp. that is the causative agent of rhinoscleroma
Klebsiella rhinoscleromatis
46
Klebsiella spp. that is implicated in human urinary tract and wound infections
Klebsiella planticola
47
Klebsiella spp. that is isolated only from the environment
Klebsiella terrigena
48
Growth of Enterobacter in EMB
Fish eye colonies
49
Most common clinical isolates of enterobacter
Enterobacter cloacae Enterobacter hormaechei
50
Rare isolate of Enterobacter spp.
Enterobacter gergoviae
51
Nonmotile Enterobacter spp.
Enterobacter asburiae
52
Enterobacter spp that is associated with osteomyelitis after traumatic wound
Enterobacter cancerogenus
53
Most common human isolates of Cronobacter
Cronobacter sakazakii Cronobacter malonaticus
54
Isolated as a pathogen in human neonates causing meningitis and bacteremia
Cronobacter sakazakii
55
Motile, slow lactose fermenter, but sucrose fermenters
Serratia spp.
56
Most significant Serratia spp.
Serratia marescens
57
Causes osteomyelitis following a motorcycle accident
Serratia plymuthica
58
Produces a very musty pungent odor resembling that of rotten tomatoes
Serratia odorifera
59
It has a major characteristic of delated positive citrate rxn
Hafnia spp.
60
None of its members ferment lactose
Proteus spp.
61
Proteus spp. that are human pathogens
Proteus mirabilis Proteus vulgaris
62
What media does it produce swarming
Sheep Blood Agar
63
Proteus spp. odor
Burnt chocolate or burnt gunpowder
64
Proteus spp that is positive for sucrose fermentation and indole
Proteus vulgaris
65
Proteus spp that is citrate and ornithine decarboxylase positive
Proteus mirabilis
66
All of its species grown on Simmons citrate medium
Citrobacter spp.
67
Citrobacter spp. that is isolated in diarrheal stool cultures
Citrobacter freundii
68
Citrobacter spp. that is the cause of nursery outbreaks of neonatal meningitis
Citrobacter koseri
69
Citrobacter spp. that is associated with community-acquired infections
Citrobacter brakii
70
Morganella spp. that is the documented cause of UTI and neonatal sepsis
Moraganella morganii
71
Providencia spp. that is a pathogen of the urinary tract
Providencia rettgeri
72
Providencia spp. that is implicated in outbreaks in burn units
Providencia stuatii
73
Providencia spp. that is most commonly found in the feces of children with diarrhea
Providencia alcalifaciens
74
Providencia spp. that is a rare isolate
Providencia rustigianii
75
Providencia spp. that is yet to be isolated
Providencia heimbachae
76
It biochemically resembles E. coli, except that this bacteria is H2S (+) and Lactose (-)
Edwardsiella spp.
77
The only oxidase-positive member of the enterobac
Plesiomonas spp.
78
Primary intestinal pathogen that does not ferment lactose
Salmonella spp.
79
Virulence factors of Salmonella
Fimbriae Enterotoxins Ability to traverse intestinal mucosa
80
Clinical Infections of Salmonella
Acute gastroenteritis or food poisoning Typhoid fever
81
Gold standard for detection of Salmonella spp.
Culture of bone marrow aspirate
82
An agglutination test which detects the presence of serum agglutinins in patients serum with suspected typhoid and paratyphoid fever
Widal Test
83
This bacteria resembles E. coli but are non-lactose fermenter
Shigella spp.
84
Pathogenicity of Shigella spp.
Shigellosis/Bacillary dysentery
85
Yersinia spp. that is the causative agent of plague
Yersinia pestis
86
Yersinia spp. that is associated with sporadic cases of gastroenteritis
Yersinia pseudotuberculosis and Yersinia enterolitica
87
Yersinia spp. that mimics appendicitis
Yersinia enterolitica
88
Optimal growth of Yersinia spp.
25-30 deg C
89
3 forms of Yersinia pestis
Bubonic Form Pneumonic Form Septicemic Form
90
This Yersinia spp. can be acquired from contact with household pets
Yersinia enterolitica
91
Selective medium of Yersinia enterolitica
Cefsulodin-irgasan-novobiocin (CIN) agar
92
Colony characteristic of Yersinia enterolitica
Bull's eye colonies
93
Yersinia spp. that is a pathogen primarily of rodents
Yersinia pseudotuberculosis