Enterobacteriaceae Flashcards
Characteristics of Enterobacteriaceae?
gram neg bacilli, oxidase neg, catalase pos
where are enterobacteriaceae found?
in soil, plants and human/animal intestines
what do enterobacteriaceae ferment
glucose + some ferment lactose
which bacteria ferment lactose?
Less dangerous pathogens ferment lactose
*Escherichia
* Klebsiella
* Enterobacter
* Citrobacter
which bacteria don’t ferment lactose
More dangerous pathogens don’t ferment lactose
Salmonella
Shigella
Yersinia
Proteus
what does ISO Stand for ?
International standardized organisation
are enterobacteriaceae fastidious?
No, they can grow anywhere
do enterobacteriaceae form spores?
No
Name 3 highly pathogenic Enterbacteriaceae?
1.Salmonella
2.Shigella
3.Yersinia
Name 6 Facultatively pathogenic
species aka
1.Escherichia coli
2.Klebsiella
3.Enterobacter
4.Proteus
5.Serratia
6.Citrobacter
what sample taken for enterobacteriaceae ?
food or faeces
what 3 agar is used for detection of Enterobacteriaceae?
- MacConkey Agar
- Violet Red Bile Glucose (VRBG) Agar
3.Hektoen or XLD Agar
What is result of enterobacteriaceae on violet red bile glucose agar?
bacteria will ferment glucose and purple colonies will form due to neutral red indicator and crystal violet
what inhibits gram pos growing on violet red bile glucose agar?
the crystal violet and bile salts
On Hektoen agar what species ferment lactose? and what color colonies grow?
e.coli- pos (so yellow colonies)
salmonella and shigella dont ferment lactose( so blue colonies )
