Enterobacteria Part 1 and 2 Flashcards
Most enterobacteria are
Are part of the body’s microflora
Salmonella and Shigella are…
Are not part of the normal microflora and they are always pathogenic
Non motile, is non lactose fermenting and does not produce H2S causes bloody diarrhea
Shigella
How does Shigella causes bloody diarrhea and uremic hemolytic syndrome and colitis?
Consists of a Shiga toxin which causes - of ribsome by removal of an amine, therefore this change results into endothelial damage
Motile, H2S production and non lactose fermenter, causes typhoid fever ?
Salmonella
Most enterobacteria are catalase + but….. except for….
oxidase -, Plesiomonas
Kleibsella consists of.. therefore can easily be stained using….?
A capsule, india ink
What is the concept of the india ink?
The india ink allows for the use of H2O in order to maintain hydration and prevent drying out
How would on test for Hyperviscosity?
Ability for the microbe to stretch > than 5 mm
Lactose fermenter but non motile and what is the reason for the non motility?
Kliebsella, because it is hyperviscous/ consists of a lot of mucoid content
Lactose fermenter and motile
E.coli
E.coli consists of beta- gluronidase enzyme, what is the function of the enzyme?
It catalyses the breakdown of large carbohydrates and allows for flourescence
The lipopolysaccharide layer consists of
O- antigen
Lipid A protein
Core polysaccharide
What are the antigenic structures that enterobacteria have?
- Heat labile K antigen
- Heat stable O antigen
- H antigen
- O antigen
- K antigen
Which O antigen is mostly pathogenic ?
O157 antigen also known as the Shiga like toxin
Which K antigens of E.coil causes disease and what disease are they associated with ?
K1- Neonatal meningitis
K- found in GI tract and Urinary Tract
Which K antigens of Kliebsella cause disease and what diseases are they associated with?
K1 & 2- Pulmonary diseases
K8, 9, 10,& 24 - Urinary Tract Infections
Where is the H antigen found?
Flagella
What is unique about the H antigen ?
Phase variation
What are colicins and pesticins?
These are bactericidal proteins.
Colicins for E.coli
Pesticins for Yersina pestis
What is the function of Bactericidal proteins?
They cause the formation of pores on the host cell therefore causing leakage of substances such as electrolytes, water. And these substances are used for production of energy therefore this results in cell death
Lipopolysaccharide is associated with what symptoms?
- Fever
- Disseminated Intravascular Coagulation
- Organ failure
- Hypotension
What are type III secretory systems?
They are known as injectasomes. They cause deliverance of toxins into the host cell
Which type of E.coli is not associated with diarrhea?
Uropathogenic E.coli
