Entamoeba histolytica Flashcards
What parasite is common in people who live in tropical areas with poor sanitary conditions?
Entamoeba histolytica
Most pathogenic amoeba; true pathogen
Entamoeba histolytica
Habitat of Entamoeba histolytica
LI - Colon
Infective stage of Entamoeba histolytica
Mature cyst (“tetra/quadrinucleated” )
Virulent stage of E. histolytica
Troph
Final host of E. histolytica
Man
MOT of E. histolytica
Ingestion of mature cyst
No. of nucleus of E. histolytica
4 (quadrinucleated)
E. histolytica cyst:
No. of nuclei
4 but It is capable of producing for nuclei but in
pratice, you may find less than four nuclei.
E. histolytica cyst:
In the nucleus, there is a dark structure which is the
_______ composed of RNA and is centrally located.
Karyosome
E. histolytica cyst:
karyosome is located _____
centrally
E. histolytica cyst:
It is on the edge of the nucleus. It is fine and smooth in appearance
peripheral chromatin
E. Histolytica Cyst Morphology:
There is also th chromatoidal body/chromidial bar/body. What is its shape?
Sausage-like, cigar -shaped
E. Histolytica Cyst Morphology:
chromatoidal body/chromidial bar/body contains what?
crystalline RNA
E. Histolytica Cyst
chromatoidal body/chromidial bar/body function
energy source
E. Histolytica Cyst:
Asie from chromatoidal body, there is also a presence of?
Glycogen vacuoles
E. Histolytica Trophozoite Morphology:
Motility - ?
progressive and unidirectional
Progressive (means it moves from one place to another)
E. Histolytica Trophozoite Morphology:
There is a presence of ____. It is described as finger-like in appearance. trophozoite releases it one at a time
Pseudopodia
E. Histolytica Trophozoite Morphology:
No. of nucleus
1
E. Histolytica Trophozoite Morphology:
Loacted of karyosome
Centrally located
E. histolytica Trophozoite Morphology:
Nucleus is surrounded by fine and smooth _____
peripheral chromatin
E. histolytica Trophozoite Morphology:
Appearance of the cytoplasm
It has a clean-looking appearance
Other important hallmarks of E. histolytica
Presence of ?
It may contain ingested RBC. The presence of RBCs indicates that you are dealing with E. histolytica.In fresh specimens (unstained preparation), RBCs appear as refractile structures that are greenish in appearance.
As a general rule, if you find cysts or trophozoites in DFS and Concentration Techniques, it is not yet conclusive. You have to to the third step in _____________ - Permanent Stained Smear
Ova and Parasite Examination
This will be used for confirmation of the presence of intestinal protozoan. This step is required for confirming intestinal protozoan.
Diagnosis?
Stain?
Permanent Stained Smear (Iron Hematoxylin or Trichrome Stain)
E. histolytica
LD used to detect antigen, and used to differ from similar parasites
Serology: ELISA
The following are the recent identification of a ‘E. histolytica look-alike”:
Entamoeba dispar
Entamoeba moshkovskii - formally known as Laredo strain - prefers to grow in 25- 30 degrees celsius
Entamoeba bangladesh
If we see any cysts or cyst stages of
entamoeba, the manner of reporting it would be
“Entamoeba histolytica/ dispar”
E. histolytica Treatment
Metronidazole/Tinidazole
Diloxanide Furoate (Especially for asymptomatic carriers)
Iodoquinol
