Entamoeba histolytica

Question 1

What parasite is common in people who live in tropical areas with poor sanitary conditions?

Answer 1

Entamoeba histolytica

Question 2

Most pathogenic amoeba; true pathogen

Answer 2

Entamoeba histolytica

Question 3

Habitat of Entamoeba histolytica

Answer 3

LI - Colon

Question 4

Infective stage of Entamoeba histolytica

Answer 4

Mature cyst (“tetra/quadrinucleated” )

Question 5

Virulent stage of E. histolytica

Answer 5

Troph

Question 6

Final host of E. histolytica

Answer 6

Man

Question 7

MOT of E. histolytica

Answer 7

Ingestion of mature cyst

Question 8

No. of nucleus of E. histolytica

Answer 8

4 (quadrinucleated)

Question 9

E. histolytica cyst:

No. of nuclei

Answer 9

4 but It is capable of producing for nuclei but in
pratice, you may find less than four nuclei.

Question 10

E. histolytica cyst:

In the nucleus, there is a dark structure which is the
_______ composed of RNA and is centrally located.

Answer 10

Karyosome

Question 11

E. histolytica cyst:

karyosome is located _____

Answer 11

centrally

Question 12

E. histolytica cyst:

It is on the edge of the nucleus. It is fine and smooth in appearance

Answer 12

peripheral chromatin

Question 13

E. Histolytica Cyst Morphology:

There is also th chromatoidal body/chromidial bar/body. What is its shape?

Answer 13

Sausage-like, cigar -shaped

Question 14

E. Histolytica Cyst Morphology:

chromatoidal body/chromidial bar/body contains what?

Answer 14

crystalline RNA

Question 15

E. Histolytica Cyst

chromatoidal body/chromidial bar/body function

Answer 15

energy source

Question 16

E. Histolytica Cyst:
Asie from chromatoidal body, there is also a presence of?

Answer 16

Glycogen vacuoles

Question 17

E. Histolytica Trophozoite Morphology:

Motility - ?

Answer 17

progressive and unidirectional
Progressive (means it moves from one place to another)

Question 18

E. Histolytica Trophozoite Morphology:

There is a presence of ____. It is described as finger-like in appearance. trophozoite releases it one at a time

Answer 18

Pseudopodia

Question 19

E. Histolytica Trophozoite Morphology:

No. of nucleus

Answer 19

1

Question 20

E. Histolytica Trophozoite Morphology:

Loacted of karyosome

Answer 20

Centrally located

Question 21

E. histolytica Trophozoite Morphology:

Nucleus is surrounded by fine and smooth _____

Answer 21

peripheral chromatin

Question 22

E. histolytica Trophozoite Morphology:

Appearance of the cytoplasm

Answer 22

It has a clean-looking appearance

Question 23

Other important hallmarks of E. histolytica

Presence of ?

Answer 23

It may contain ingested RBC. The presence of RBCs indicates that you are dealing with E. histolytica.In fresh specimens (unstained preparation), RBCs appear as refractile structures that are greenish in appearance.

Question 24

As a general rule, if you find cysts or trophozoites in DFS and Concentration Techniques, it is not yet conclusive. You have to to the third step in _____________ - Permanent Stained Smear

Answer 24

Ova and Parasite Examination

Question 25

This will be used for confirmation of the presence of intestinal protozoan. This step is required for confirming intestinal protozoan.

Diagnosis?
Stain?

Answer 25

Permanent Stained Smear (Iron Hematoxylin or Trichrome Stain)

Question 26

E. histolytica
LD used to detect antigen, and used to differ from similar parasites

Answer 26

Serology: ELISA

Question 27

The following are the recent identification of a ‘E. histolytica look-alike”:

Answer 27

Entamoeba dispar
Entamoeba moshkovskii - formally known as Laredo strain - prefers to grow in 25- 30 degrees celsius
Entamoeba bangladesh

Question 28

If we see any cysts or cyst stages of
entamoeba, the manner of reporting it would be

Answer 28

“Entamoeba histolytica/ dispar”

Question 29

E. histolytica Treatment

Answer 29

Metronidazole/Tinidazole
Diloxanide Furoate (Especially for asymptomatic carriers)
Iodoquinol