energy transfers in and between organisms P2 Flashcards
what is the role of light in photoionisation in the light dependent stage of photosynthesis
chlorophyll molecules absorb energy from photons of light. this Excites the electrons (raising their energy level) causing them to be released (lost) from the chlorophyll (and go down ETC)
what are the 2 main stages involved in ATP production in light-dependent reaction?
electron transfer chain
chemiosmosis
what happens in the electron transfer chain?
electrons released from chlorophyll move down a series of carrier proteins embedded in thylakoid membrane and undergo a series of redox reactions which releases energy
how is proton concentration gradient established during chemiosmosis?
some energy released from ETC is coupled to active transport of protons from stroma into thylakoid space
how does chemiosmosis produce ATP in light-dependent reaction?
protons move down concentration gradient from thylakoid space into stroma through ATP synthase.
ATP synthase catalyses ADP+Pi—>ATP
explain the role of light in photolysis
light energy splits water molecules into protons, electrons and oxygen
what happens to the products of photolysis of water?
protons move out of thylakoid space via ATP synthase and are used to reduce the coenzyme NAPD
electrons replace electrons lost from chlorophyll
oxygen is used for respiration and diffuses out of leaf as waste gas
how and where is reduced NADP produced in the light dependent reaction?
NADP + proton + electron —> reduced NADP
catalysed by dehydrogenase enzymes
in stroma of chloroplasts
where do the H+ ions and electrons to reduce NADP come from?
H+ ions - photolysis of water
electrons - NADP acts as the final electron acceptor of the electron transfer chain
describe what happens during photoionisation in the light-dependent reaction
chlorophyll absorbs light, which excites elections causing electrons to be lost.
name the three main stages in the calvin cycle
carbon fixation
reduction
regeneration
what happens during carbon fixation?
reaction between carbon dioxide and RuBP (ribulose biphosphate)
forms unstable 6 carbon molecule (2x GP)
what happens during reduction in the calvin cycle?
2 x GP is reduced to 2 x TP (triose phosphate) which requires reduced NADP and ATP for energy.
forms NADP and ATP
how does the light- independent reaction result in the production of useful organic substances
one carbon leaves the cycle
6 cycles to make glucose
what happens during regeneration in the calvin cycle?
after 1C leaves the cycle, 5C is used to regenerate RuBP using ATP
(ATP—>ADP +Pi)
state the roles of ATP and NADPH in the light independent reaction
ATP: reduction of GP to TP and provides energy to regenerate RuBP
NADPH: coenzyme transports electrons needed for the reduction of GP to TP
describe the structure of a chloroplast
usually disc shaped, double membrane
thylakoids - flattened discs stack to form grana
lamellae- tubular extensions attach thylakoids in adjacent grana
stroma - fluid-filled matrix
how does the structure of the chloroplast maximise the rate of the light dependent reaction?
ATP synthase channels within granal membrane
large surface area of thylakoid membrane for electron transport chain.
photosystems position chlorophyll to enable maximum absorption of light
how does the structure of the chloroplast maximise the rate of the light independent reaction?
own DNA and ribosomes for synthesis of enzymes (rubisco)
concentration of enzymes and substrates in stroma is high.
define limiting factor
factor that determines maximum rate of a reaction, even if other factors change to become more favourable
name 4 environmental factors that could limit the rate of photosynthesis
light intensity (light dependent stage)
CO2 levels (light independent stage)
temperature (enzyme controlled steps)
mineral/ magnesium levels (maintain normal functioning of chlorophyll)
outline some common agricultural practices used to overcome the effect of limiting factors in photosynthesis
artificial light, especially at night
artificial heating
addition of CO2 to greenhouse atmosphere
why do farmers try to overcome the effect of limiting factors?
to increase yield
additional cost must be balanced with yield to ensure maximum profit
as light is increased, it then plateaus. explain why
all NADP has become reduced from the non-cyclic photophosphorylation (light-dependent)
as CO2 concentration increases, so does photosynthesis up to a point. why does it stop increasing?
no more RuBP available as all combines with CO2 or no more rubisco (all have formed ES complexes)
as temperature increases, so does photosynthesis, up to a point. explain why it stops increasing
changed to tertiary structure of ATP synthase/ rubisco