Energy Transfers 3.5 Flashcards
Photosynthesis (AO1)
Which reaction comes first in photosynthesis?
Light-dependent
Photosynthesis (AO1)
During photosynthesis, plants produce ____________ compounds which contain carbon, such as carbohydrates, lipids and proteins.
organic
Photosynthesis (AO1)
Most of the sugars synthesised by plants during photosynthesis are used by the plant in ____________ .
The rest are used to make other groups of biological molecules.
respiration
Photosynthesis (AO1)
Chlorophyll location
Thylakoid membrane
(found in chloroplasts).
Photosynthesis (AO1)
What process do photoautotrophic organisms use to convert carbon dioxide into organic molecules?
Photosynthesis
Photosynthesis (AO1)
Examples of photoautotrophic organisms
plants
algae
Photosynthesis (AO1)
Light-dependent reaction location
Thylakoid membrane
(found in chloroplasts).
Photosynthesis (AO1)
What molecule’s electrons are excited / released during photoionisation?
Chlorophyll
Photosynthesis (AO1)
TRUE or FALSE:
Chlorophyll absorbs all wavelengths of light
FALSE
The pigment ‘chlorophyll a’ does NOT absorb green light, so reflects it
Photosynthesis (AO1)
What is the term for the splitting of water by light energy into protons, electrons and oxygen?
Photolysis
Photosynthesis (AO1)
Which chemicals are needed for the light-dependent reaction
NADP, ADP, Pi and water
Photosynthesis (AO1)
Describe photoionisation
(2 marks).
1. Chlorophyll absorbs light energy
OR light energy excites / releases electrons from chlorophyll;
2. Electron/s go to the electron transport chain
Photosynthesis (AO1)
Molecules located in the thylakoid membranes
Chlorophyll
Electron transport chain proteins
(includes channel protein for protons)
ATP Synthase
(includes channel protein for protons)
Photosynthesis (AO1)
Products of the light-dependent reaction
NADPH and ATP
Reduced NADP is accepted.
Photosynthesis (AO1)
Describe what happens during the light-dependent reaction (5 marks).
- Chlorophyll absorbs light energy;
- Excites electrons / electrons removed (from chlorophyll);
- Electrons move along electron transport chain releasing energy;
- Energy used to join ADP and Pi to form ATP;
- Photolysis of water produces protons, electrons and oxygen;
- NADP reduced by electrons and protons
Photosynthesis (AO1)
______________ enters most ecosystems through the light-dependent reaction of photosynthesis.
Energy
Photosynthesis (AO1)
Describe the role of chlorophyll in photolysis (3 marks).
Absorbs light energy;
Loses electrons;
Accepts electrons from water;
Photosynthesis (AO1)
Describe how NADP is reduced in the light-dependent reaction of photosynthesis (2 marks).
by electrons and protons from photolysis;
by electrons from chlorophyll;
Photosynthesis (AO1)
What molecule is a nitrogen-containing biological molecule, a type of nucleotide, and has two phosphate groups?
NADP
This is useful knowledge for the essay!
Photosynthesis (AO1)
What process involves the movement of protons down an electrochemical gradient to produce ATP?
Chemiosmosis
Photosynthesis (AO1)
The light independent reaction is also known as the __________ cycle
Calvin
Photosynthesis (AO1)
Light independent reaction location
Stroma
Photosynthesis (AO1)
Rubisco location
Stroma
Photosynthesis (AO1)
Describe the function of NADPH in the light-independent reaction.
Provides H / electrons for the reduction of GP to triose phosphate
Photosynthesis (AO1)
Describe the function of ATP in the light-independent reaction (1 mark).
Provides energy for the reduction of GP to triose phosphate;
Provides Pi / inroganic phosphate to convert triose phosphate to RuBP;
Photosynthesis (AO1)
Describe the light-independent reaction of photosynthesis (6 marks).
- Carbon dioxide combines/reacts with ribulose bisphosphate/RuBP;
- Produces two GP molecules using (enzyme) Rubisco;
- GP reduced to triose phosphate;
- Using reduced NADP / NADPH;
- Using energy from ATP hydrolysis;
- Triose phosphate converted to glucose / RuBP / named organic substance;
Photosynthesis (AO1)
Describe how the products of the light-dependent reaction are used in the light-independent reaction to produce triose phosphate (3 marks).
- ATP and reduced NADP / NADPH;
- GP reduced to triose phosphate;
- ATP provides energy;
Photosynthesis (AO3)
Environmental factors that should be controlled when measuring the rate of photosynthesis
- Light intensity
- Carbon dioxide concentration
- Temperature
- Water OR humidity
- Wavelength of light
Note: which factors are controlled depends on the IV of the experiment
Photosynthesis (AO1)
Limiting factors for photosynthesis
Temperature
Carbon dioxide concentration
Light intensity
Wavelength of light
Photosynthesis (AO3)
Explain why scientists measured the rate of production of oxygen when investigating the rate of photosynthesis (2 marks).
Oxygen is produced in the light dependent reaction;
The faster the oxygen produced, the faster the light dependent reaction;
Photosynthesis (AO2)
Heat stress is a condition that often occurs in plants exposed to high temperatures for a prolonged period of time. Heat stress is a major factor in limiting the rate of photosynthesis.
Heat stress decreases the light-dependent reaction of photosynthesis.
Explain why this leads to a decrease in the light-independent reaction (2 marks).
- Less ATP;
- Less reduced NADP / NADPH;
Photosynthesis (AO2)
Atrazine is a herbicide used to reduce the growth of weeds.
Atrazine binds to proteins in the electron transfer chain in chloroplasts of weeds, reducing the transfer of electrons down the chain.
Explain how this reduces the rate of photosynthesis in weeds (4 marks).
- Reduced transfer of protons across thylakoid membrane
OR
Reduced chemiosomotic gradient / proton gradient across thylakoid membrane;
- (So) less ATP produced;
- (So) less reduced NADP / NADPH produced;
- (So) less reduction of GP to triose phosphate.
OR
(So) light-independent reaction slows / stops;
Photosynthesis (AO2)
Iron deficiency reduces electron transport. Use this information and your knowledge of photosynthesis to explain the decrease in production of triose phosphate in the iron-deficient plants (4 marks).
- ATP and reduced NADP produced during light-dependent reaction;
(In the case of iron deficiency)
- Less ATP produced;
- Less reduced NADP produced;
- Less GP to triose phosphate;
Photosynthesis (AO2)
Some bacteria use hydrogen sulfide, H2S, to produce organic compounds. The hydrogen sulfide has a similar role to that of water in photosynthesis.
A simple equation for this process in bacteria is shown below:
hydrogen sulfide + carbon dioxide → glucose + sulfur + water
Suggest what the hydrogen sulfide is used for in these bacteria (2 marks).
- (Provides) hydrogen/protons/H+ and electrons/e-;
- For reduction (of NADP);
- Source of electrons for chlorophyll / electron transfer chain;
Photosynthesis (AO2)
Another effect of heat stress is a decrease in the activity of the enzyme rubisco. A decrease in the activity of an enzyme means that the rate of the reaction it catalyses becomes slower.
A decrease in the activity of the enzyme rubisco would limit the rate of photosynthesis.
Explain why (2 marks).
- Less carbon dioxide (reacts) with RuBP;
- Less GP;
Photosynthesis (AO2)
An investigation was carried out into the effect of carbon dioxide concentration and light intensity on the rate of photosynthesis in a species of plant.
The temperature was kept constant during this investigation. Explain why (2 marks).
Temperature affects photosynthesis;
because it affects enzyme activity;
So that any change in photosynthesis rate is result of carbon dioxide / light intensity;
Photosynthesis (AO2)
Data from an investigation showed that mutant plants producing more chlorophyll b grow faster than normal plants in all light intensities.
Explain how (4 marks).
- Have faster production of ATP and reduced NADP;
- (So) have faster / more light-independent reaction;
- (So) produce more sugars that can be used in respiration;
- (So) have more energy for growth;
- Have faster / more synthesis of new organic materials (e.g. amino acids).
Respiration (AO1)
Respiration produces ………………………
ATP
Respiration (AO1)
Aerobic respiration stages
1. Glycolysis
2. Link reaction
3. Krebs cycle
4. Oxidative phosphorylation
Respiration (AO1)
TRUE OR FALSE:
Glycolysis is the first stage of anaerobic AND aerobic respiration
TRUE
Respiration (AO1)
Glycolysis location
Cytoplasm
Respiration (AO1)
Describe glycolysis (4 marks)
1. Phosphorylation of glucose using ATP;
2. Oxidation of triose phosphate to pyruvate;
3. Net gain of (2) ATP (molecules);
4. NAD reduced/ NADH produced;
Respiration (AO1)
Dehyrogenase action during glycolysis
Removes hydrogen from triose phosphate so oxidised to pyruvate
Adds hydrogen to NAD so reduced to NADH
Respiration (AO1)
During glycolysis, ATP is produced via ____________-____________ phosphorylation
substrate-level
Respiration (AO1)
Net yield of 2 ATP during glycolysis
2 ATP used to phosophorylate glucose
4 ATP produced during substrate level phosphorylation (triose phosphate to pyruvate)
Respiration (AO1)
TRUE or FALSE:
Anaerobic respiration produces more ATP than aerobic respiration
False
Lots of ATP is produced during oxidative phosphorylation
Respiration (AO1)
If respiration is aerobic, pyruvate from glycolysis enters the mitochondrial matrix by…………………….
active transport
Respiration (AO1)
TRUE or FALSE:
Glucose crosses the double membrane of mitochondria via active transport
FALSE
Respiration (AO1)
Link reaction location
(Mitochondrial) matrix
Respiration (AO1)
Fill in the blanks:
During the first step of the link reaction pyruvate is oxidised to [1] and the removed hydrogen is used to reduce [2]. During this step, carbon [3] is also released/removed by the enzyme [4].
- acetate
- NAD
- dioxide
- decarboxylase
Respiration (AO1)
Carbons in pyruvate
3 carbons
Respiration (AO1)
Carbons in acetate
2 carbons
Respiration (AO1)
A - pyruvate
B - reduced NAD / NADH
C - coenzyme A
D - AcetylcoenzymeA
Respiration (AO1)
Decarboxylase action during link reaction
removes carbon during oxidation of pyruvate, releasing carbon dioxide and resulting in the formation of acetate
Respiration (AO1)
Describe how acetylcoenzyme A is formed in the link reaction (2 marks)
1. Oxidation of / hydrogen removed from pyruvate AND carbon dioxide released;
2. Addition of coenzyme A (to 2C acetate).
Respiration (AO1)
Link reaction reactants
Pyruvate
NAD
CoenzymeA
Respiration (AO1)
Acetylcoenzyme A reacts with a four-carbon molecule, releasing coenzyme A and producing a six-carbon molecule that enters the ……………………..
Krebs cycle
Respiration (AO1)
Krebs cycle location
(Mitochondrial) matrix
Respiration (AO1)
The Krebs cycle is a series of oxidation-reduction reaction that produces………
Products through oxidation-reduction:
Reduced NAD / NADH
Reduced FAD / FADH
other products:
ATP by substrate-level phosphorylation
Carbon dioxide
A 4C molecule
Respiration (AO1)
Importance of the 4C product from the Krebs cycle
Allows the Krebs cycle to continue;
So more NADH and FADH produced;
So more ATP can be produced during oxidative phosphorylation
Respiration (AO1)
Enzyme that produces NADH and FADH
Dehydrogenase
Respiration (AO1)
Enzyme that produces carbon dioxide during the link reaction and Krebs cycle
Decarboxylase
Respiration (AO1)
TRUE OR FALSE:
FADH is produced during the link reaction and Krebs cycle
FALSE
FADH is only produced during the Krebs cycle
Respiration (AO1)
Draw out the Krebs cycle
Respiration (AO1)
Respiration (AO2)
Malonate inhibits a reaction in the Krebs cycle.
Explain why malonate would decrease the uptake of oxygen in a respiring cell (2 marks).
1. Less/no reduced NAD/ reduced FAD
2. Oxygen is the final/terminal (electron) acceptor;
Less NADH/FADH = less electron transfer, so less oxygen required.
Respiration (AO1)
What do NADH and FADH bring to the electron transport chain?
(High energy) electrons