Energy Transfers (3.5) Flashcards
This topic covers: photosynthesis, respiration, energy transfers and nutrient cycles.
AS Recap (Gas Exchange)
Explain the relationship between stomatal opening and photosynthesis (2 marks).
- Stomata allow uptake of carbon dioxide;
- Carbon dioxide is required for photosynthesis
AS Recap (Gas Exchange)
Describe how carbon dioxide in the air outside a leaf reaches mesophyll cells inside the leaf (3 marks).
- Carbon dioxide enters via stomata;
- (Stomata opened by) guard cells;
- Diffuses through air spaces (in the spongy mesophyll layer);
- Down diffusion/concentration gradient;
AS Recap (Cells)
Which of the below plant cell organelles has stacked membranes arranged in parallel and contains DNA. (1 mark).
Cell Wall
Chloroplast
Nucleus
Mitochondrion
Golgi apparatus
Chloroplast
AS Recap (Cells)
Give one feature of the chloroplast that allows protein to be synthesised inside the chloroplast and describe one difference between this feature in the chloroplast and similar features in the rest of the cell (2 marks).
Mark in pairs, 1 and 2 OR 3 and 4
- Feature: DNA;
- Difference: Is not associated with protein/histones but nuclear DNA is;
OR Is circular but nuclear DNA is linear
OR Is shorter than nuclear DNA;
- Ribosomes;
- Are smaller than cytoplasmic ribosomes;
Accept: 70S ribosomes in chloroplast, but 80S ribosomes in cytoplasm
AS recap (Cells)
Where is chlorophyll located in a plant mesophyll cell (1 mark)?
Thylakoid membrane (of granum) found in chloroplasts.
A2 (Photosynthesis AO1)
What process do photoautotrophic organisms use to convert carbon dioxide into organic molecules (1 mark)?
Photosynthesis
A2 (Photosynthesis AO1)
Which reaction comes first in photosynthesis (1 mark)?
Light-dependent
A2 (Photosynthesis AO1)
What molecule’s electrons are elevated to a higher energy state during photoionisation (1 mark)?
Chlorophyll
A2 (Photosynthesis AO1)
Where does the light-dependent reaction take place within the plant mesophyll cell (1 mark)?
Thylakoid membrane
A2 (Photosynthesis AO1)
What is the term for the splitting of water into H+, e- and oxygen (1 mark)?
Photolysis
A2 (Photosynthesis AO1)
What waste product is generated as a result of photolysis of water (1 mark)?
Oxygen
A2 (Photosynthesis AO1)
Which chemicals are needed for the light-dependent reaction? (1 mark)
NADP, ADP, Pi and water
A2 (Photosynthesis AO1)
Describe what happens during photoionisation in the light-dependent reaction (2 marks).
- Chlorophyll absorbs light energy
OR Light excites/moves electrons in chlorophyll;
- Electron/s are lost
Accept electrons go to electron transport chain for ‘electrons lost’.
A2 (Photosynthesis AO1)
What are the products of the light-dependent reaction? (1 mark)
NADPH and ATP
Reduced NADP is accepted.
A2 (Photosynthesis AO1)
Energy enters most ecosystems through the light-dependent reaction of photosynthesis.
Describe what happens during the light-dependent reaction (5 marks).
- Chlorophyll absorbs light energy;
- Excites electrons / electrons removed (from chlorophyll);
- Electrons move along electron transport chain releasing energy;
- Energy used to join ADP and Pi to form ATP;
- Photolysis of water produces protons, electrons and oxygen;
- NADP reduced by electrons and protons
A2 (Photosynthesis AO1)
Describe the role of chlorophyll in photolysis (3 marks).
Absorbs light energy;
Loses electrons;
Accepts electrons from water
A2 (Photosynthesis AO1)
Describe how NADP is reduced in the light-dependent reaction of photosynthesis (2 marks).
by electrons;
from chlorophyll / photolysis
A2 (Photosynthesis AO1)
What molecule is a nitrogen-containing biological molecule, a type of nucleotide, and has two phosphate groups? (1 mark)
NADP
A2 (Photosynthesis AO1)
What process involves the movement of protons down their gradient to produce ATP? (1 mark)
Chemiosmosis
A2 (Photosynthesis AO1)
Where does the Calvin cycle take place? (1 mark)
Stroma
Therefore the enzyme rubisco is located in the stroma.
A2 (Photosynthesis AO1)
Describe one function of NADPH in the light-independent reaction (1 mark).
Provides H / electrons for the reduction of GP to triose phosphate
You must say triose phosphate in full!
A2 (Photosynthesis AO1)
Describe one function of ATP in the light-independent reaction (1 mark).
Provides energy for the reduction of GP to triose phosphate;
Provides Pi to convert triose phosphate to RuBP;
A2 (Photosynthesis AO1)
Describe the light-independent reaction of photosynthesis (6 marks).
- Carbon dioxide combines/reacts with ribulose bisphosphate/RuBP;
- Produces two GP molecules using (enzyme) Rubisco;
- GP reduced to triose phosphate;
- Using reduced NADP / NADPH;
- Using energy from ATP hydrolysis;
- Triose phosphate converted to glucose/hexose/RuBP/ribulose bisphosphate/named organic substance;
A2 (Photosynthesis AO2)
Heat stress is a condition that often occurs in plants exposed to high temperatures for a prolonged period of time. Heat stress is a major factor in limiting the rate of photosynthesis.
Heat stress decreases the light-dependent reaction of photosynthesis.
Explain why this leads to a decrease in the light-independent reaction (2 marks).
- Less ATP;
- Less reduced NADP / NADPH;
A2 (Photosynthesis AO2)
Atrazine is a herbicide used to reduce the growth of weeds.
Atrazine binds to proteins in the electron transfer chain in chloroplasts of weeds, reducing the transfer of electrons down the chain.
Explain how this reduces the rate of photosynthesis in weeds (4 marks).
- Reduced transfer of protons across thylakoid membrane
OR
Reduced chemiosomotic gradient / proton gradient across thylakoid membrane;
- (So) less ATP produced;
- (So) less reduced NADP / NADPH produced;
- (So) less reduction of GP to triose phosphate.
OR
(So) light-independent reaction slows / stops;
A2 (Photosynthesis AO2)
Iron deficiency reduces electron transport. Use this information and your knowledge of photosynthesis to explain the decrease in production of triose phosphate in the iron-deficient plants (4 marks).
- ATP and reduced NADP produced during light-dependent reaction;
(In the case of iron deficiency)
- Less ATP produced;
- Less reduced NADP produced;
- Less GP to triose phosphate;
A2 (Photosynthesis AO2)
Some bacteria use hydrogen sulfide, H2S, to produce organic compounds. The hydrogen sulfide has a similar role to that of water in photosynthesis.
A simple equation for this process in bacteria is shown below:
hydrogen sulfide + carbon dioxide → glucose + sulfur + water
Suggest what the hydrogen sulfide is used for in these bacteria (2 marks).
- (Provides) hydrogen/protons/H+ and electrons/e-;
- For reduction (of NADP);
- Source of electrons for chlorophyll / electron transfer chain;
A2 (Photosynthesis AO2)
Another effect of heat stress is a decrease in the activity of the enzyme rubisco. A decrease in the activity of an enzyme means that the rate of the reaction it catalyses becomes slower.
A decrease in the activity of the enzyme rubisco would limit the rate of photosynthesis.
Explain why (2 marks).
- Less carbon dioxide (reacts) with RuBP;
- Less GP;
A2 (Photosynthesis AO2)
Plants produce ATP in their chloroplasts during photosynthesis. They also produce ATP during respiration.
Explain why it is important for plants to produce ATP during respiration in addition to during photosynthesis (5 marks).
- In the dark no ATP production in photosynthesis in leaves;
- Some tissues (e.g. roots) unable to photosynthesise / produce ATP;
- ATP cannot be moved from cell to cell / stored
(as it is an immediate energy compound); - Plant uses more ATP than produced in photosynthesis;
- For example, ATP required for active transport / protein synthesis;
A2 (Photosynthesis AO2)
An investigation was carried out into the effect of carbon dioxide concentration and light intensity on the rate of photosynthesis in a species of plant.
The temperature was kept constant during this investigation. Explain why (2 marks).
Temperature affects photosynthesis;
because it affects enzyme activity;
So that any change in photosynthesis rate is result of carbon dioxide / light intensity;
A2 (Photosynthesis AO1)
List potential limiting factors for photosynthesis
Temperature
Carbon dioxide concentration
Light intensity
Wavelength of light
AS Recap (Cells)
Identify organelles S and T
S = Vacuole
T = Chloroplast
AS Recap (Cells)
Identify structures D and E
D = Grana/granum/thylakoid
E = Starch/lipid
AS Recap (Cells)
B;
A;
E;
AS Recap (ATP)
Describe how an ATP molecule is formed from its component molecules (4 marks).
- and 2. Accept for 2 marks correct names of three components adenine, ribose, three phosphates;;
- Condensation (reaction);
- (catalysed by) ATP synthase;
A2 (Photosynthesis AO2)
Data from an investigation showed that mutant plants producing more chlorophyll b grow faster than normal plants in all light intensities.
Explain how (4 marks).
- Have faster production of ATP and reduced NADP;
- (So) have faster / more light-independent reaction;
- (So) produce more sugars that can be used in respiration;
- (So) have more energy for growth;
- Have faster / more synthesis of new organic materials (e.g. amino acids).
A2 (Photosynthesis AO2)
During an investigation into the rate of photosynthesis.
Explain why scientists measured the rate of production of oxygen in this investigation (2 marks).
Oxygen is produced in the light dependent reaction
The faster the oxygen produced, the faster the light dependent reaction.
AS Recap (ATP)
Draw out and label a single molecule of ATP
P = phosphate, not phosphorus.
Which organelle is shown in the below TEM?
Mitochondrion
Singular, plural = mitochondria
AS Recap (ATP)
ATP is useful in many biological processes. Explain why (4 marks).
- Releases energy in small / manageable amounts;
- (Broken down) in a one step / single bond broken OR immediate energy compound / makes energy available rapidly;
- Phosphorylates / adds phosphate makes (phosphorylated substances) more reactive / lowers activation energy;
- Do not accept phosphorus or P on its own
- Rapidly reformed / made again;
- Is not lost from / does not leave cells.
AS Recap (Cells)
Identify X
Cristae
Part of the folds of the inner mitochondrial membrane
AS Recap (Cells)
AS Recap (Cells)
Give the function of a mitochondrion.
The site of aerobic respiration (reactions)
OR
ATP is made / ADP is phosphorylated;
A2 (Respiration AO1)
Respiration produces ………………………
ATP
A2 (Respiration AO1)
TRUE OR FALSE:
Glycolysis is the first stage of anaerobic AND aerobic respiration
TRUE
A2 (Respiration AO1)
Where does glycolysis take place?
Cytoplasm
A2 (Respiration AO1)
Describe the process of glycolysis (4 marks).
1. Phosphorylation of glucose using ATP;
2. Oxidation of triose phosphate to pyruvate;
3. Net gain of (2) ATP (molecules);
4. NAD reduced/ NADH produced;
A2 (Respiration AO1)
If respiration is aerobic, pyruvate from glycolysis enters the mitochondrial matrix by…………………….
active transport
Pyruvate therefore crosses the double membrane of mitochondria
A2 (Respiration AO1)
Where does the link reaction take place?
(Mitochondrial) matrix
A2 (Respiration AO1)
Pyruvate is oxidised to ……………….. during the link reaction.
Acetate
This produces carbon dioxide and NADH
A2 (Respiration AO1)
Describe how acetylcoenzyme A is formed in the link reaction (2 marks)
1. Oxidation of / hydrogen removed from pyruvate AND carbon dioxide released;
2. Addition of coenzyme A (to 2C acetate).
A2 (Respiration AO1)
What are the reactants needed for the link reaction?
Pyruvate
NAD
CoenzymeA
A2 (Respiration AO1)
Acetylcoenzyme A reacts with a four-carbon molecule, releasing coenzyme A and producing a six-carbon molecule that enters the ……………………..
Krebs cycle
A2 (Respiration AO1)
Where does the Krebs cycle take place?
(Mitochondrial) matrix
A2 (Respiration AO1)
The Krebs cycle is a series of oxidation-reduction reaction that produces………
Products through oxidation-reduction:
Reduced NAD / NADH
Reduced FAD / FADH
other products:
ATP by substrate-level phosphorylation
Carbon dioxide
A2 (Respiration AO1)
What is the name of the enzyme that helps to produce NADH and FADH?
Dehydrogenase
A2 (Respiration AO1)
What is the name of the enzyme that helps to produce carbon dioxide during the link reaction and Krebs cycle?
Decarboxylase
A2 (Respiration AO1)
A2 (Respiration AO1)
TRUE OR FALSE:
FADH is produced during the link reaction and Krebs cycle
FALSE
FADH is only produced during the Krebs cycle
A2 (Respiration AO1)
Malonate inhibits a reaction in the Krebs cycle.
Explain why malonate would decrease the uptake of oxygen in a respiring cell.
- Less/no reduced NAD/ reduced FAD OR
Fewer/no hydrogens/electrons removed (and passed to electron transfer chain);
- Oxygen is the final/terminal (electron) acceptor;
Less NADH/FADH = less electron transfer, so less oxygen required.
A2 (Respiration AO1)
Describe how ATP is made in mitochondria (6 marks).
Substrate level phosphorylation
- ATP produced in Krebs cycle;
Oxidative phosphorylation
- Krebs cycle and link reaction produces NADH;
Krebs cycle produces FADH
- Electrons released from NADH / FADH;
- (Electrons) pass along carriers / through electron transport chain / through series of redox reactions;
- Energy released;
- Protons move into intermembrane space (electrochemical / proton gradient forms);
(Protons move down electrochemical gradient and release energy used to combine)
- ADP / ADP + Pi;
- ATP synthase