Energy transfer in and between organisms Flashcards
What are the adaptations of leaves in order to carry out photosynthesis most effectively?
- Large surface area to absorb sunlight
- Leaf arrangements to stop overlapping of leaves and shadowing of each other
- Thin for a short diffusion distance
- Transport cuticle/epidermis to let light through to the mesophyll layer
- Long upper mesophyll cells with many chloroplasts for sunlight
- Many stomata for gas exchange/short diffusion path
- Stomata responds to differences in light intensity (open/close)
- Air spaces in lower mesophyll layer for rapid diffusion
- Xylem + Phloem network to bring water/carry sugars produced
What is the balanced symbol equation for photosynthesis?
6CO2 + 6H20 -> C6H12O6 + 6O2
What is the word equation for photosynthesis?
Carbon Dioxide + Water -> Glucose + Oxygen
What are the 3 stages of photosynthesis?
- Capturing light energy -> by chloroplast pigments (e.g chlorophyll)
- Light Dependent Reaction -> light energy is conserved in chemical bonds, an electron flow is created by effect of light on chloroplast pigments. Products are reduced NADP, ATP and Oxygen
- Light Independent Reaction -> Protons are used to produce sugars and organic molecules
What is the structure of chloroplasts?
Disc shaped + 2-10 micrometres long + double membrane
Grana (stacks of thylakoids) where LDR happens
Sroma (fluid filled matrix) where LIR happens
What is the light dependent reaction of photosynthesis?
Chlorophyll absorbs light energy causing the photoionisation of chlorophyll
Some energy from the electrons released during photoionisation is conserved in the production of ATP and reduced NADP
ATP production involves electrons moving down an electron transfer chain and the movement of protons across chloroplast membrane down a concentration gradietn and this is catalysed by ATP synthase - Chemiosmotic Theory
The photolysis of water produces protons, electrons and oxygen
What is the equation for the photolysis of water?
H20 -> 2H+ + 2e- + 1/2O2
Water -> Protons + Electrons + Oxygen
What are the products of the LDR?
ATP and NADPH
How do protons cross the phospholipid bilayer between thylakoids and stroma?
Protons are pumped via proton carriers from stroma
Electrons transfer energy from photolysis of water and proteins increase their concentration
Concentration gradient between thylakoids and stroma of proteins is maintained (high -> thylakoid, low -> stroma)
Protons can only cross membrane through ATP synthase channel proteins (rest of membranes is hydrophobic so protons are repelled)
As protons pass through ATP synthase channel, enzyme structure is changed causing the catalysis of ADP + Pi to lower activation energy to form ATP.
Protons pass out of thylakoids through ATP synthase channels, NADPH is formed from NADP - electron carrier - taking up protons
What is the Light independent reaction?
Uses NADPH from LDR to form a simple sugar, hydrolysis of ATP provides additional energy
What occurs in the light independent reaction? (Calvin Cycle)
- CO2 diffuses into the stroma
- Co2 reacts with RuBP and this reaction is catalysed by Rubisco which produces two molecules of 3 carbon GP
- NADPH is used to reduce GP to Triose Phosphate using energy from ATP
- NADP is reformed and goes back
- Some triose phosphate is used to regenerate RuBP in calvin cycle
What are the products of the Light Independent Reaction?
RuBP and Oxygen
What is the site of the independent reaction?
Takes place in the stroma of the chloroplasts
What are the limiting factors of photosynthesis?
- Light Intensity
- CO2
- Temperature
Why is Light Intensity a limiting factor of photosynthesis?+ describe the shape of the graph
Curve that has a steep gradient at first but eventually levels off
Steep gradient -> more light therefore more photons hitting photosystems thus more photoionisation, more electrons excited and more ATP available for the LIR and more reduced NADP. More photolysis releases more O2
Levels off-> Limited amount of ADP/NADP available, if they run out there is nothing to accept electrons so LDR gets blocked