Embryonic Development Flashcards
First trimester
Embryonic period
Weeks 1-8
Second Trimester
Fetal period
Weeks 9-birth
Fertilization Calendar
Ovulatory calendar, starts once the egg and the sperm meet (fertilization is initiated
Obstetrical calendar
Gestational calendar, menstrual calendar
Clinical calendar used, starts at the first day of the females LMP,
- 2 weeks longer than menstrual calendar
Corona radiata
Cells from ovary (maternal)
Zona pellucida
Acellular glycoprotein mesh work surrounding egg
Nucleus
Haploid cell, 23 chromosomes including (XX)
Plasma membrane
Cell membrane of egg cell
Pervitelline space
Between zona pellucida and the cell membrane of the egg
Acrosome
Covers anterior 2/3 of head, contains enzymes to help sperm erode cell layers surrounding egg
Anatomy of Human sperm
Head
Neck
Tail
Tail
Middle piece
Principle Piece
End Piece
Middle piece
Contains mitochondria for motility
Fertilization
Day 0
Occurs in fallopian tube, specifically the ampula
Week 1
Day 0-7 Fertilization Formation of zygote Formation of blastocyst Implantation into the uterine wall
** Formation of inner cell mass
Ampula
Distal 2/3 of fallopian tube where egg and sperm first meet
Capacitation
Glycoprotein coat and seminal plasma proteins are removed from plasma membrane of sperm
- occurs in uterus and uterine tubes
- takes approximately 7 Hours to complete
Acrosome reaction
Follows capacitation, sperm is ready to move through layers
Hydraluronidase
Enable passage of sperm through corona radiata
Penetration of Zona pellucida
- Esterases, acrosine, and neuraminidase are secreted to help break through Zona pellucida
Zona reaction
Once one sperm makes it in, no others can come in
- block to polyspermy
Plasma membrane fusion
Of oocyte and sperm, head an tail of sperm enter oocyte cytoplasm, Mitochondria do NOT
Completion of second meiotic division
- formation of male pronucleus
* fusion of male and female pronuclei -> zygote
Results of fertilization
- completion of 2nd meiotic division
- restoration of diploid number of chromosomes
- determination of chromosomal sex of embryo
- metabolic activation of oocyte
- initiation of cleavage
Blastomeres
Cells once embryo is beginning to divide
Cleavage
Increase in cell number, decrease in cell size, as size is regulated by zona pellucida
- starts ~30 hours after fertilization
- Embryo size is unchanged
Morula is developed
Morula
16-32 cells, formed at ~ 3 days, enters uterus at ~ day 4
Early blastocyst
Zona pellucida degenerating
Pluripotent cells formed, (stem cells)
Late blastocyst
Zona pelucida gone, embryo is one cell layer, and inner cell mass (stem cell)
Trophoblasts
Stem cells of the placenta
Differentiates into cytotrophoblast and syncytiotrophoblast
Embryonic stem cells
- derived from the inner cell mass
- Pluripotent- form virtually any cell or tissue type
- therapeutic cloning and somatic nuclear transfer
Day 6
Implantation of trophoblast