Embryo physiology, morphology and ET

Question 1

In vivo what temperature do embryos grow at?

Answer 1

37 degrees

Question 2

In vivo what pH do embryos grow at?

Answer 2

7.2-7.4

Question 3

In vivo what oxygen concentration do embryos grow at?

Answer 3

low

Question 4

In vivo what is the metabolic profile of the oviduct fluid which embryos develop in?

Answer 4

high pyruvate and lactate
low glucose

Question 5

In vivo what is the metabolic profile of the uterine fluid which embryos develop in?

Answer 5

high glucose
low pyruvate and lactate

Question 6

How is a good embryo measured?

Answer 6

through growth rate and morphology

Question 7

Morphology is an easy and quick assessment of predicting which embryos have the highest chance of resulting in pregnancy. However the method has its limitations, what are to consider in respect to morphology as a tool for determining a good embryo?

Answer 7

morphology and genetics are not well correlated

Morphological features have proven no biological significance

embryologists are not trying to make embryos better but just trying to not stress them out

Question 8

On a day 1 fertilisation check at 17 hpi, what are you looking for?

Answer 8

2 pronuclei (dents in the oocyte)

2 polar bodies

Question 9

On a day 1 fertilisation check at 23 hpi, what are you looking for?

Answer 9

0 pronuclei (only if two were seen at 17 hpi)

2 polar bodies