embedding

Question 1

arrangement of the tissue in a precise position in the mold during embedding

Answer 1

orientation

Question 2

all layers in a transverse section

Answer 2

Tubular tissue

Question 3

all layers should come

Answer 3

skin and intestine

Question 4

keep in center

Answer 4

endometrial curetting

Question 5

keep diagonally

Answer 5

long tissue

Question 6

swiss roll

Answer 6

membrane

Question 7

types of molds

Answer 7

1. Leuckhart’s embedding mold
2. Compound embedding unit
3. Plastic embedding rings and base mold
4. Disposable embedding molds

Question 8

Disposable embedding molds

Answer 8

1. Peel away
2. Plastic ice tray
3. Paper boat

Question 9

Tissues are first infiltrated w/ celloidin and subsequently

Answer 9

DOUBLE-EMBEDDING METHOD

Question 10

For high resolution light microscopy of thinner than usual sections; renal biopsies, BM biopsies

Answer 10

PLASTIC RESIN EMBEDDING

Question 11

types of PLASTIC RESIN EMBEDDING

Answer 11

(APE)
Acrylic plastic
Polyester
Epoxy

Question 12

> Most widely applied, but carcinogenic due to vinylcyclohexane dioxide (VCD)
component,
For electron microscopy

Answer 12

epoxy

Question 13

types of epoxy

Answer 13

BGC
> bisphenol A (araldite) - slow
> Glycerol (epon) - low viscosity
> cyclohexane dioxide (spurr) - very low viscosity, fastest

Question 14

For electron microscopy; seldom used

Answer 14

Polyester

Question 15

> For high resolution microscopy
Acrylic plastics must be stored in dark bottles to prevent radical formation and
premature polymerization.
Eg: polyglycol methacrylate (GMA), methyl methacrylate (MMA)
Embedding media may be stained, thus use hydrophobic MMA.

Answer 15

Acrylic plastic

Question 16

A catalyst; forms radicals, which are a site
for polymerization

Answer 16

benzoyl peroxide