ELISA Test

Question 1

What does the ELISA test allow you to identify?

Answer 1

If a patient has any antibodies to a certain antigen or any antigen to a certain antibody

Question 2

What is the ELISA test used to test in medical diagnosis?

Answer 2

Pathogenic infections

Question 3

In the ELISA test, why is an antibody with an enzyme attached used?

Answer 3

Enzyme can react with a substrate to produce a coloured product causing the solution in the reaction vessel to change colour

Question 4

What does a colour change demonstrate in an ELISA test?

Answer 4

The antigen or antibody of interest is present in the sample being tested

Question 5

What is the simplest type of ELISA?

Answer 5

Direct ELISA

Question 6

What does a direct ELISA use?

Answer 6

A single antibody that is complementary to the antigen being tested for

Question 7

How is indirect ELISA different to direct ELISA?

Answer 7

Indirect uses 2 different antibodies whereas direct only uses 1

Question 8

What are the ethical issues surrounding the use of monoclonal antibodies?

Answer 8

Animal rights issues