ELISA TEST Flashcards
1
Q
What does the ELISA test stand for?
A
Enzyme - Linked - ImmunoSorbent - Assay
2
Q
What are put onto a slide/in a well?
A
Antigens from the sample of blood/urine/saliva
3
Q
What antibodies do we add to the antigen?
A
Specific to the antigens and we leave them to bind
4
Q
Why do we wash the slide/well inbetween steps?
A
To remove unbound antigens and antibodies and enzymes to prevent false positives
5
Q
What does the second molecule we add onto the antigen-antibody complex consist of?
A
2nd antibody with enzymes attached to it which is complimentary to the 1st antibody
6
Q
What kind of substrate do we add?
A
A colourless substrate
7
Q
In what kind of result of the enzymes change the colour of the substrate?
A
In a positive result
