ELISA and Lateral Flow Essays

Question 1

[2-minute video]: Direct ELISA

Answer 1

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Question 2

[2-minute video]: Indirect ELISA

Answer 2

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Question 3

[2-minute video]: Sandwich ELISA

Answer 3

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Question 4

[2-minute video]: Competitive ELISA

Answer 4

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Question 5

ELISA in full

Answer 5

Enzyme-Linked Immunosorbent Assay

Question 6

4 types of ELISA

Answer 6

✔ Direct ELISA
✔ Indirect ELISA
✔ Sandwich ELISA
✔ Competitive ELISA

Question 7

Briefly describe Direct ELISA.

Answer 7

The antigen is immobilized on a plate, and the enzyme-linked antibody binds directly to the antigen. Upon addition of the substrate, a measurable signal (color change) occurs.

Question 8

Briefly describe Indirect ELISA.

Answer 8

The antigen is immobilized on a plate, and a primary antibody binds to the antigen. A labeled secondary antibody specific to the primary antibody is then added, followed by substrate to produce a signal.

Question 9

Briefly describe Sandwich ELISA.

Answer 9

The capture antibody is immobilized on a plate and binds to the antigen. The detection antibody, which is enzyme-linked, then binds to the antigen. Addition of the substrate generates a signal.

Question 10

Briefly describe Competitive ELISA.

Answer 10

The sample antigen and a labeled antigen compete for binding to an antibody immobilized on a plate. The more antigen present in the sample, the less labeled antigen binds to the antibody. A lower signal indicates higher antigen concentration.