ELISA ADVANTAGES AND DISADVANTAGES

Question 1

ADVANTAGE:

High ___ and ___ : it is common for ELISAs to detect antigens at the ___ lin a very specific manner due to the use of antibodies.

Answer 1

Sensitivity , specificity

Picogram level

Question 2

ADVANTAGE:

High ___: commercial ELISA kits are normally available in a ___ format.

But the assay can be easily adapted to ___.

Answer 2

throughput

96-well plate format

384-well plates

Question 3

ADVANTAGE:

___: Protocols are easy to follow and involve little hands-on time.

Answer 3

Easy to perform

Question 4

ADVANTAGE:

____: It can determine the CONCENTRATION of ___ in a sample.

Answer 4

Quantitative

Antigen

Question 5

ADVANTAGE:

Possibility to test VARIOUS SAMPLE TYPES: ___, ___, ___ and ___, ___ and ___ among others.

Answer 5

serum, plasma, cellular and tissue extracts, urine, saliva

Question 6

DISADVANTAGE:

___: Detection is based on ___ and therefore (prior answer) must be obtained in a ___.

Answer 6

Temporary readouts

Enzyme/Substrate reactions

readouts

Short time span

Question 7

____: It is limited to the amount or presence of the ____ in the sample.

Answer 7

Limited antigen information

antigen