ELISA

Question 1

What does ELISA stand for?

Answer 1

Enzyme-linked immunosorbent assay

Question 2

What is ELISA used to detect?

Answer 2

Widely used laboratory technique used for detecting and quantifying the presence of specific substances such as:

1.Antigens

2.Antibodies

3.Hormones

Question 3

What is the process of an antigen ELISA

Answer 3

1. Antibodies are stuck onto a multi well plate, and left for some time to allow it to adhere to the well.
2. A sample antigen from the virus being tested for is added, they will stick to the antibodies.
3. Next, a second antigen with a marker is added and a positive reaction is detected by the marker changing colour when an appropriate substrate is added.
4. If there are no antigen in the sample, the second antigen will not be able to stick and there will be no colour change.

Question 4

What are the benefits for ELISA

Answer 4

Sensitivity and specificity