Electrophysiology of the visual system Flashcards
When a photoreceptor becomes hyper-polarised (in light), how is the response transmitted throughout the photoreceptor to the synapse with the bipolar cell?
There is calcium influx along the length of the photoreceptor which is released at the synapse with Bipolar cells along with glutamate
What is an M (magnocellular) cell?
Large ganglion cell
What is a P (Parvocellular) cell?
Small ganglion cell
At which synapse are horizontal cells located?
At the synapse between photoreceptors and bipolar cells
At which synapse are amacrine cells present?
At the synapse between bipolar cells and ganglion cells
What percentage of all cones are long-medium (red-green wavelength) cones?
85%
How many cones must be stimulated for colour detection?
At least two, for wavelength discrimination
How is colour discriminated in the retina?
The stimulation of a blue (short wavelength cone) is compared to stimulation of a red-green (long) wavelength cone
Which photoreceptor detects dim light?
Rods
Which photoreceptor detects dim light?
Rods
Which photoreceptor detects bright light?
cones
Describe how rods connect to a ganglion cell
Rods connect indirectly to ganglion cells via cone bipolar cells
Multiple rods synapse with rod-bipolar cell which connects Amacrine II cells which connects to cone-bipolar cell which connects to ganglion cell
How are horizontal cells involved in isolating a stimulated photoreceptor?
Horizontal cells send a inhibitory stimulus to ganglion cells surrounding the stimulated photoreceptors
Are amacrine cells more numerous or horizontal cells?
Amacrine cells
How many types of amacrine cells are there?
29 types
Name some of the neurotransmitters used by Amacrine cells to modify the ganglion cell response.
Dopamine Glutamate Glycine Acetylcholine GABA
How many types of ganglion cells are there?
15
What is the resting potential at the interface between the RPE and photoreceptors?
60mV
what is the electro-oculogram?
It is a record of the electrical dipole occurring between the front and back of the eye.
How is the current on a electro-oculogram reversed?
By moving the back and forth in horizontal direction
How does bright illumination affect the height of the potential difference in a electro-oculogram?
Bright light increases the height of the potential difference
When is the electro-oculogram lost?
In condition that disrupt the apposition of the RPE with the photoreceptor e.g. retinal detachment
What is the electro-retinogram (ERG)?
Cumulative electrical response to light stimulus from all the retinal elements
Where does the early receptor potential originate?
It originates from the photochemical reactions in the rod outer segments upon stimulation by light
What is the early receptor potential dependent on?
- density of rods
2. high levels of unbleached rhodopsin
What does the ERG begin with?
Early receptor potential
Is the early receptor potential normally recorded?
No
Is the early receptor potential recordable when the inner retina is destroyed but the outer retina is intact e.g. central retinal artery occlusion?
Yes
What are the different waves of the electroretinogram (ERG)?
- a waves
- b waves
- oscillatory potentials
What does the a wave in the ERG represent?
Hyperpolarisation of the photoreceptors inner segments
Is the a wave in the ERG negative or positive?
negative
Is the a1 wave in ERG for rods or cones?
cones
Is the a2 wave in the ERG for rods or cones?
rods
What does the b wave in the ERG represent?
bipolar cells
Is the b1 wave in ERG from cone or receptor bipolar cells?
cone
Is the b2 wave in the ERG from cone or rod bipolar cells?
Rods
What pathology causes the b wave to be lost in the ERG?
retinal vascular conditions e.g. central retinal vein occlusion
What do the oscillatory potentials in the ERG represent?
amacrine cells
In what condition are oscillatory potentials lost in an ERG?
diabetes
What is visual evoked potential (VEP)?
Records the electrical activity in the visual cortex following exposure to light stimulus
How is a VEP recorded?
6 electrodes are placed around the left and right occipital cortices