Electrophysiology Flashcards

1
Q

Volts generated by animals, such as electric eels or electric rays

A

700 volts

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2
Q

Volts in negative resting membrane potential

A

about -70 millivolts

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3
Q

Small variable changes in the membrane potential which are in the range of one to 40 millivolts.

A

Post-synaptic potentials

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4
Q

How are action potentials?

A

They are large, fast, and occur in an all-or-none fashion.

They reach 100 millivolts.

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5
Q

At rest, sodium-potassium exchanges use _____to alter the distribution of the ions across the membrane.

A

ATP

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6
Q

What similarity does electroplaques and neurons have?

A

They both have sodium-potassium exchanges in their plasma membrane

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7
Q

Volts in resting membrane potential

A

-60 and -80 millivolts inside the neuron

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8
Q

Which ions are inside the cell?

A

Chloride and sodium

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9
Q

Which neurotransmitter evoke electric discharge in electroplaques?

A

Acetylcholine

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10
Q

Number of sodium and potassium molecules exchanged in the Na+/K- ATPase

A

3 Na+ exit the cell and 2 K- enter the cell

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11
Q

Number of chloride and potassium molecules exchanged in the potassium chloride cotransporter

A

1 Cl- and 1 K- exit the cell

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12
Q

In Vmrest, which ions enter the cell by electrical gradient?

A

Ca+, K+ and Na+

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13
Q

In Vmrest, which ions enter the cell by concentration gradient?

A

Ca+, Cl- and Na+

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14
Q

In Vmrest, which ions exit the cell by electrical gradient?

A

Cl-

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15
Q

In Vmrest, which ions exit the cell by concentration gradient?

A

K+

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16
Q

Potentials generated by activation of ion channels that depolarize neurons

A

Excitatory postsynaptic potentials EPSP

17
Q

Potentials generated by activation of ion channels that hyperpolarise neurons

A

Inhibitory postsynaptic potentials IPSP

18
Q

Characteristics of EPSP and IPSP

A

Graded in amplitud due to concentration of neurotransmitter and length of the time the neurotransmitter is in the synaptic cleft

Additive but decay in amplitud as they move around the neuron

19
Q

Name state of Na channels in each stage of the curve of the action potential stages

A

Resting Vm. Closed resting

Upstroke. Open

Peak. Closed inactivating

Downstroke. Closed inactivated

Resting Vm Close resting

20
Q

Name state of Na channels in each stage of the curve of the action potential stages

A

Resting Vm. Closed resting

Upstroke. Closed resting

Peak. Start opening

Downstroke. Open

Resting Vm Close resting

21
Q

Which types of potentials can be recorded from outside the cell?

A

Excitatory potentials and action potentials

22
Q

Which type of potentials can be generated by neurotransmitter ion channel receptors ?

A

Excitatory potentials and postsynaptic potentials

23
Q

Which type of potential can be generated by voltage gated ion channels ?

A

Action potentials

24
Q

Types of electrophysiologic recording

A

Extra cellular

Intracelular

Single channel recording

25
Q

What is a gigaseal ?

A

Seal that is formed between the patch-clamp electrode with the cell’s surface and interact with the membrane.

26
Q

What is an on-cell Patch clamp?

A

Gigaseal with the cell membrane which measures single channel activity using a micropipette

27
Q

How do we do an inside out patch and what does it measure?

A

Pull the patch of membrane off the cell and have access to the intracelular side of the patch

We can apply drugs and still record single channel activity

28
Q

Name the versions of extracellular recordings

A

Field potentials

Whole nerve recordings

Multicellular recordings

Multi-unit recordings

Single unit recordings

MEAs

29
Q

What cannot be recorded with extracellular recording methods?

A

Membrane potentials

Vm rest

Postsynaptic potentials

30
Q

Difference between current clamp and voltage clamp

A

Current clamp “clamp” the cell potential at a chosen value. Measures ionic current at any given voltage

Voltage clamp records membrane potential. The membrane potential is free to vary.

31
Q

How is a whole cell clamp made?

A

We break the membrane with the electrode still attached to the whole cell

32
Q

Usefulness of whole cell clamp

A

Enables us to record from all ion channels in the membrane of a particular cell (whole cell recording)

33
Q

How to made an outside out patch ?

A

Pull away the electrode from a whole cell patch pulling off a small patch of membrane exposing the outside of the membrane to the outside environment.

34
Q

What is a perforated patch?

A

Diffuse an antibiotic to the membrane and forms pores

35
Q

Usefulness of the perforated patch

A

You don’t lose things from the inside the cell and still get electrical activity