electrophoresis

Question 0

what is a plasmid

Answer 0

small circular double stranded Dna molecule

Question 1

what is transformation?

Answer 1

taking genetic info and putting it in another and able to express it

Question 2

what living organism was in p glo lab? why?

Answer 2

E. coli bacteria multiplies quick easy to grow

Question 3

what is an operon? how was it used?

Answer 3

cluster of genes that are all related. cut out genes from dna and replaced it with gene from jellyfish

Question 4

how do u know if pglo was transformed?

Answer 4

if it glowed and growed

Question 5

how could antibiotic resistance affect bacteria?

Answer 5

they could get used to it and gain resistance to it.

Question 6

real world uses for transformation and dna prints

Answer 6

crime scene, baby daddy, pharmacutical products, hebicide resistant crops, genetic disease

Question 7

how could antibiotic resistance affect other organisms?

Answer 7

if you’re sick you could be resistant to the antibiotic

Question 8

hot water bath?

Answer 8

opens up holes in plasmid

Question 9

calcium chloride?

Answer 9

pokes holes in bacterial cell wall

Question 10

Ice?

Answer 10

close up holes and trap plasmid

Question 11

how does bacteria live?

Answer 11

food-lb agar warmth- incubator darkness- incubator moisture- closed Petri dish

Question 12

3 parts of a nucleotide?

Answer 12

sugar phosphate n base

Question 13

gel electrophoresis?

Answer 13

separate dna fragments by size

Question 14

genetic code of DNS is determined by what?

Answer 14

nitrogen bases

Question 15

why do we stain gel?

Answer 15

to see DNa because it’s clear

Question 16

why do we use loading dye?

Answer 16

to know when to turn off gel box so fragments font run out into buffer

Question 17

gates down before gel box?

Answer 17

current can’t go through plastic

Question 18

dna is what charge

Answer 18

negative

Question 19

wells are on what side of box?

Answer 19

cathode negative move toward anode postive bc dna is negative

Question 20

segment of dna on chromosome that controls hereditary trait?

Answer 20

gene

Question 21

pyrimidines?

Answer 21

C T

Question 22

purines?

Answer 22

A G

Question 23

why do we change pipete tips?

Answer 23

so we don’t contaminate

Question 24

enzymes that cut?

Answer 24

restriction enzymes

Question 25

scientist who came with base pairing rules

Answer 25

chargaff

Question 26

function of DNa ?

Answer 26

store and use info to control cell activity

Question 27

acid or base test?

Answer 27

phenol red… yellow=acid red/pink=base

Question 28

2 scientists who won Nobel prize for dna structure?

Answer 28

Watson and crick

Question 29

2 things b4 power supply ?

Answer 29

everything plugged in correctly, announce ur turning it on

Question 30

dna nucleotide has what?

Answer 30

deoxyribose, thymine, phosphate group

Question 31

what side was acid and base?

Answer 31

yellow was anode positive pink was cathode negative

Question 32

what were in + tubes?

Answer 32

hind 3 dna loading dye buffer water

Question 33

why did we add nacl?

Answer 33

to add ions to produce a current (milliamps)