Electron Transport Chain Flashcards
What is the name of complex 1?
NADH-Q oxidoreductase
What is the name of complex 2 ?
Succinate-Q reductase
What is the name of complex 3?
Q-cytochrome c oxidoreductase
What is the name of complex 4?
Cytochrome- c oxidase
What does FMN stand for and what do the reduced form carry?
FMN- Flavins
In their reduced form they carry hydrogen( proton plus an electron)
What does FeS stand for, what do they carry and what residues are they bound to ?
FeS= iron- sulphur cluster, bound to cysteine residues and carry electrons as they are capable of undergoing oxidation and reduction
What is the difference between ubiquinone and ubiquinol?
ubiquinone is the oxidised form of coenzyme Q and ubiquinol is the reduced form of coenzyme Q. They carry electrons and protons.
Which out of Q, FMN and FeS can act as a mobile carrier?
Q due to it’s lipophillic nature
What is the overall reaction in NADH-Q oxidoreductase?
NADH + Q + 5H+ -> NAD+ + QH2 + 4 H+
Q- cytochrome c oxidoreductase is made up of which subunits ?
Riske iron sulphur protein, cytochrome b and cytochrome c1 both cytochromes are to carry hemes. Has heme bL and bH
Is cytochrome c covalently or non covalently attached ?
covalently
Cytochrome a and b are non covalently attached.
in which complex does the proton motive Q- cycle take place and give a brief description?
in complex 3
one electron goes from Q0- FeS- Cyt c1- Cyt c
and the other goes from Q0-Cyt bL- Cyt bH to Q1
what is the overall reaction of complex 3?
Qh2 + 2 cyt c (oxidised + 2 H+ -> Q + 2 Cyt c (reduced) + 4 H+ to cystol
Complex 3 leaves you with how many reduced Cyt C’s?
2 Cyt C’s and 1 reduced QH2.
Cytochrome c oxidase contains which redox active components?
Heme a, heme a3, and Cua/Cua which is copper and Cub
Describe the route of electrons from cytochrome c to CuB.
Cytochrome c- Cua/Cua- Heme a- Heme a3 - CuB
What does o2 bind to in cytochrome c oxidase ?
Reduced Fe in heme a3
what is the name of the bridge formed from the transfer of electrons to O2?
Peroxide bridge
What is the overall reaction of Complex 4?
4 Ctyt C + 4H+ + O2 -> 4Cyt c +2 H2O
How many additional protons can complex 4 pump across the membrane?
4 from the matrix the the inter membrane space.
The F0 component of ATPase is in the matrix?
No it is located in the membrane and contains a ring of hydrophobic proteins.
how many alpha and beta subunits is F1 made up of?
3 alpha and 3 beta
which subunit is the catalytic and which is the regulatory in the ATP synthase?
alpha is the regulatory and beta is the catalytic
How many subunits does the c ring have in vertebrates and in one rotation how many ATP’s are made?
The c ring contains 8 subunits and in one rotation 3 ATP’s are made
what formation does the O, L and T stand for of the alpha and beta subunits of the ATP synthase
0= open and releases ATP T= tight and L= loose which binds ADP and Pi.
what is the P: O ratio for NADH and FADH
NADH- 2.7 and FADH- 1.6.
NADH= 10(protons)/ 3.7( H+ per ATP made) whereas FADH= 6(H+)/ per 3.7( same)
