Electron Microscopy Flashcards

1
Q

Recall - electron microscopy

A

Electron microscopes have a much higher resolution than light microscopes because they use electrons rather than light.

This means they are highly useful in imaging sub cellular compartments, for example, contractile, machinery of myocytes proteins and other macromolecules.

They can also be used to show the structure of viruses.

Slides for electron microscopes require more complex preparation

They are very useful for linking macromolecular structure and function and have helped in understanding the cell

Transmission electron microscope have a resolution of 10 to 0.5 nm into magnification of times 1 million.

The electron beam penetrates a section and electrons are absorbed by any material they meet, which, then, in tracts with a phosphorescent screen

This type of microscopy requires thin plastic resin section of around 50 to 100 nm thick

Electron microscopes have a resolution of to 2,0.2 nm on a magnification of times 100,000.

A thicker sample can be used as a surface is scanned by a probe with electrons, interacting with sample to cause secondary electrons to balance of the sample, which are then detected and shown on a screen

Extension

Samples are stained with heavy metal salts, for example, uranium, or lead in transmission microscopy

Scanning, electron microscope’s are used to study the surface of cells and organelles

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