Effect of sucrose concentration on pollen tube growth Flashcards

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1
Q

Independent variable

A

sucrose concentration

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2
Q

Dependent variable

A

Length of pollen tube in given time

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3
Q

Variables to control

A

Temperature
Maturity of flower providing pollen
O2 availability

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4
Q

Advantages of using pollen from ONE flower

A
  1. Improves validity of results because the maturity of the flower is controlled

This means any differences in results are more likely to be a result of sucrose concentration.

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5
Q

Disadvantages of using pollen from ONE flower

A

Different results may arise if the experiment is repeated

Flowers may vary slightly in maturity

Genetic differences

Ideally the investigation would be repeated with a large number of flowers ( calculate mean)

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6
Q

Why do you add a moistened filter paper in the Petri dish?

A

The humidity in the dish stops the pollen from DRYING out

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7
Q

Why is there no coverslip on the slide?

A

To avoid ANOXIC conditions

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