EDNA:

Question 1

What are the advantages of eDNA?

Answer 1

Non-invasive sampling: allows for detection of species without the need to physically observe or capture them
Ability to detect a wide range of species simultaneously
Taxonomic expertise is not required

Question 2

What are the limitations to eDNA barcoding?

Answer 2

1. Bioinformatic skills required
2. Contamination risks

Question 3

What is the strategy to overcome contamination in eDNA samples?

Answer 3

Using PPE to minimise human contamination
Conducting PCR in dedicated rooms to avoid cross-contamination
Including negative controls in experiments to identify potential contamination sources

Question 4

What is the strategy to bioinformatics speciality?

Answer 4

• user friendly software
• collaborations with bioinformatics specialists
• online analysis platforms