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Hilary week 1 > EBV part 1 > Flashcards

EBV part 1 Flashcards

1
Q

Which herpes virus family does EBV belong to? And what other herpes virus is in this family?

A

gamma herpesviruses

-also includes karposis sarcoma associated herpesvirus.

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2
Q

What is the structure of the EBV virus?

A

glycoprotein envelope, protein tegument and nucleocapsid containing large dsDNA

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3
Q

how is EBV transmitted and what is the incubation period?

A

transmitted via saliva

4-6 week incubation period.

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4
Q

Which cells does EBV infect? Which cells is latency established in?

A

Infects B cells and epithelial cells

Latency established in B memory cells

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5
Q

What can happen if infection occurs during adolescence?

A

Infectious mononucleosis.

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6
Q

What are the 4 symptoms of mono?

A

fatigue, fever, sore throat, lymphadenopathy

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7
Q

What two complications can arise with mono?

A

splenomegaly, hepatic involvement

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8
Q

Which glycoprotein is involved in EBV infection of B cells? And what receptor on B cells does it bind?

A

gp350, binds to CD2/ CD21 on B cells.

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9
Q

What proteins make up the core fusion machinery of EBV and what do they bind to.

A

gp42, gH/gL and gB.

Initial binding of the former to HLA II in endosomes.

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10
Q

describe the two steps of B cell EBV infection.

A
  1. gp350-CD2/21 receptor-mediated endocytosis.

2. gp42, gH gL gB binding to HLA II in endosomes triggers membrane fusion and nucleocapsid release.

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11
Q

What receptors are likely targeted for epithelial cell EBV infection? How many stages?

A

integrin receptors likely targeted (avB6/8).

Only one direct step.

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12
Q

What parts of the core fusion machinery is required for epithelial cell infection?

A

gH, gL and gB.

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13
Q

Which structure of the tonsils (oropharyngeal lymphoepithelial barrier) does EBV target?

A

the epithelial cells lining the tonsilar crypts and the local circulating B cells.

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14
Q

What kind of B cell infection is established first?

A

establishes an initial latency III infection, producng growth transformed B cells.

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15
Q

What three groups of EBV proteins expressed in latent III, what effect on B cells?

A

EBNA1, 2, 3 (A,B,C) and LP.
LMP1 and 2.
BHRF1

As a result B cells are blasted and immortalised.

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16
Q

What kind of viral protein is BHRF1?

A

an anti-apoptotic protein.

17
Q

What form does the latent EBV genome take?

A

circular genome.

18
Q

What route can some of the growth transformed B cells take?

A

Some will enter a lytic cycle, that enable viral replication and shedding.

19
Q

Gives examples of viral genes that are expressed sequentially through lytic progression.

A

Immediate early- TFs like BZLF1 (for expression of others)
Early, DAN replication proteins e.g. BMLF1
LAte- encodes structural proteins e.g. BLLF1

20
Q

What general groups can EBV antigens belong to?

A

latent and lytic infection.

21
Q

What are three major cellular responses against lytic primary infection?

A

NK cell potentially, and large CD8+ T cel response as well as a CD4+ response.

22
Q

What primary responses are made against latent infection?

A

CD8 and CD4 responses.

23
Q

What kind of infection is established following the primary immune response against EBV?

A

The Lat0 infection - where gene expression is switched off to evade immune recognition.

24
Q

What is the only gene that is expressed during latent ) and what does it do?

A

EBNA1 only present in small amounts- tether viral EBV genome to host chromosome.

25
Q

What can happen to these Lat0 cells?

A

They will periodically activate into the lytic cycle to repopulate latent B cell pool (asymptomatically)

26
Q

T cells are vital for control of EBV, what happens to immunosuppressed people?

A

malignancy of transformed B cells.

27
Q

Immunosuppressed EBV B cell malignancies? Which stages of infection is it associated with?

A

post transplant lymphoproliferativ disease
Hodgkin lymphoma
Burkitt lymphoma
diffuse lare B cell lymphoma

28
Q

What two epithelail cell malignancies with EBV?
Whats the rare lymphoma affecting other cell populations?

What proteins associated with?

A

nasopharyngeal carcinoma and gastric carcinoma.

extranodal NK/T cell lymphoma

29
Q

PTLD and DLBCL associated proteins?
Hodkin and nasopharyngeal carcinoma?
Burkitt?

A

All the proteins expressed in latency III
Latnecy II EBNA1, and LMP1 and 2 (A/B)
EBNA1 (Lat 1/0)

Hilary week 1 (5 decks)

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