EBV Flashcards
EBV presents with on a slide with; in clinic with:
atypical lymphocytes, lymphadenopathy, hepatosplenomegaly
after detecting EBV on a slide, the next step is:
heterophil test (monospot test)
infectious mononucleosis patients develop antibodies called:
heterophil antibodies
a positive ______ test has:
heterophil, red agglutinations on a blue backgound
a negative _____ test has_____
heterophil, homogenous and purple suspension
in a ____ test, you mix ___ with ____
heterophil monospot test, patient serum, horse or sheep RBC
a ______ is unique and a diagnostic indicator of EBV
heterophil antibody
the heterophil test is both ___ and ____, but is _____ for younger patients
sensitive and specific, less (more false negatives)
after a heterophil test, you _____
serologic test for capsid antigen of EBV
a EBV serologic test indicates levels of:
VCA-IgM, VCA-IgG,, EA, EBNA
in an acute infection, the ebv serologic test is:
VCA-IgM +, VCA-IgG +, EA +/-, ,EBNA-,
in a past infection, the ebv serologic test is:
VCA-IgM - , VCA-IgG+, EA-, EBNA+
EBV induces proliferation of _____ in the _____ which resemble____
malignant lymphocytes (B cells)
EBV infects_____ in the _____, but the atypical cells you see in patient’s blood are _____
B cells, lymph nodes, cytotoxic T cells (which are not infected but rather are responding to the infection)
EBV initially infects___ then spreads to _____
oropharyngeal epithelial cells, underlying lymphoid tissue (tonsils and adenoids where mature B cells are infected)
what is the B cell receptor that allows it to bind EBV?
CD21
2 phases of EBV infection?
lytic (proliferation/replication, infection, and lysis of B cells) latency (most exist as an extrachromosomal episome)
the EBV genes are associated with:
the pathogenesis of latency
the EBV genes we need to know are:
EBNA1, 2, LMP1, vIL-10
EBNA stands for____ and what does it do?
Epstein-Barr nuclear antigen 1, 2, bind the EBV genome to the host cell chromosomes during mitosis, promotes B cell replication
LMP1 stands for___ and what does it do?
latent membrane protein, dries b-cell activation, prevents apoptosis
what does vIL-10 do?
suppresses cell-mediated responses
disease process of EBV?
Infectious Mononucleousis, primary disease process off EBV, and is less severe the younger the patient is (may present as a head cold or stuffy nose)
b cells activated by EBV secrete _____ antibodies
heterophile antibodies (autobodies that recognize sheep or horse rbcs), or autoantibodies (anti-platelets of your own cells leading to thrombocytopenia)
What 2 cellular responses occur in response to EBV
B cells: secrete heterophile antibodies that recognize sheep or horse RBCs, autoantibodies that opsonize platelets
T cells: CD8+ cytotoxic and NK cells control proliferation lymphocytes (these are NOT infected with EBV but are responding to the infected B cells)
clinically, EBV resembles (overlaps with), need to be careful of _____ injury
malignancy, splenic rupture (fatal)
atypical lymphocytosis are ___ cells that are _____:
CD8 cytotoxic Tcells and NK cells, not infected with EBV
3 groups of herpes viruses
alpha (hsv-1, 2, VZV) neurons, beta (CMV, HHV6), gamma EBV, HHV8-Kaposi’s) latency in lymphoid cells
CMV histologically is known for____ and can disseminate causing
large inclusions surrounded by a clear halo “owl’s eyes”; “looks like an alien looking at you”; disseminate PNU,
acute transient viral infection:
elicit effective immune responses that eliminate the pathogens, limiting the durations of the infections
measles (rubeola) presents with:
koplik spots (blue bumps inside cheek), “warthin-Finkeldey” multi-nucleated cells in lung and sputum
warthin-finkeldey cells signal:
measles (rubeola)
Poliovirus is:
acute transient viral infection, enterovirus transmitted via the fecal-oral route, infects anterior horn of spinal cord
in the serology of an EBV reactivation, what two levels are elevated?
VCA-IgG, ENBA IgG
immunosenescence:
gradual deterioration of the immune system brought on by natural age advancement; decreased cell-mediated immunity (T-cell functino) can cause reactivation of EBV, major increase in age related mortality
