E3L4: Cell Signaling 2

Question 1

GPCR subtype Alpha-(1A) is part of which principal G-protein? A. Gq B. Gi C. Go D. Gs E. Two of the above

Answer 1

A.

Question 2

GPCR subtype Alpha-(1B) is part of which principal G-protein? A. Gq B. Gi C. Go D. Gs

Answer 2

A.

Question 3

GPCR subtype Alpha-(1D) is part of which principal G-protein? A. Gq B. Gi C. Go D. Gs

Answer 3

A.

Question 4

GPCR subtype Alpha-(2A) is part of which principal G-protein? A. Gq B. Gi C. Go D. Gs

Answer 4

B and C

Question 5

GPCR subtype Alpha-(2B) is part of which principal G-protein? A. Gq B. Gi C. Go D. Gs

Answer 5

B and C

Question 6

GPCR subtype Alpha-(2C) is part of which principal G-protein? A. Gq B. Gi C. Go D. Gs

Answer 6

B and C

Question 7

GPCR subtype Beta-1 is part of which principal G-protein? A. Gq B. Gi C. Go D. Gs

Answer 7

D

Question 8

GPCR subtype Beta-2 is part of which principal G-protein? A. Gq B. Gi C. Go D. Gs

Answer 8

D

Question 9

GPCR subtype Beta-3 is part of which principal G-protein? A. Gq B. Gi C. Go D. Gs

Answer 9

B, C, and D

Question 10

Which of the following are involved in maintaining LOW cytosolic calcium?

a. An antiporter on the PM
b. A P-type pump on the PM
c. A P-type pump on the ER
d. Calcium binding molecule
e. A P-type pump on the mitochondria

f. All of the above

Answer 10

f. All of the above

Question 11

What is true about the effect of vasopressin?

A. It increases the magnitude of Ca2+ influx into the cell
B. It increases the magnitude of the Ca2+ efflux into the cell
C. It increases the frequency of intracellular Ca2+ oscillations
D. It decreases the frequency of intracellular Ca2+ oscillations

Answer 11

C. It increases the frequency of intracellular Ca2+ oscillations

Question 12

Which of the following can auto-phosphorylate? Circle all that apply:

A. CaM-Kinase II
B. PDGF receptor

C. Adenylyl Cyclase

D. PKA

Answer 12

A. CaM-Kinase II
B. PDGF receptor

TRICKY: PDGF receptor is a tyrosine kinase receptor!

Question 13

Which of the following is NOT activated by calmodulin?

A. eNOS
B. nNOS
C. CaM-Kinase II

D. PKG

Answer 13

D. PKG

Question 14

Bradykinin binds GPCRs on endothelial cells, which effect _______ principal G-proteins

a.Gq

b. Gi
d. Go
e. Gs

Answer 14

a. Gq

Question 15

State the events of NO-mediated vascular relaxation in correct order.

Answer 15

1. Bradykinin binds GPCR
2. Gq activates PLC
3. PLC cleaves PIP2, producing IP3
4. Ca is released from the ER
5. Calmodulin binds NOS
6. NO diffuses to the smooth muscle
7. NO binds soluble guanylyl cyclase (sGC)
8. sGC creates cGMP from GTP
9. cGMP activates PKG, which promotes muscle relaxation