E. coli calculation formulas Flashcards
1
Q
Transformation efficiency
A
Total number of colonies growing on the LB/amp per μg of plasmid DNA used for the transformation
2
Q
Initial amount or mass of plasmid DNA
A
Total amount of DNA (μg) = concentration (μg/μl) X volume (μl)
3
Q
Fraction of DNA solution spread
A
Volume of solution spread on plate divided by total amount in tube = fraction spread
4
Q
Mass of DNA spread on the experimental plate
A
Total mass of DNA [μg] X fraction spread = Mass of DNA spread
5
Q
Number of colonies per μg of plasmid DNA
A
Number of colonies observed divided by mass of DNA spread = Transformation efficiency
