Drugs and the Cardiovascular System – The Heart Flashcards
What is the major store of calcium within the cardiomyocyte?
Sarcoplasmic reticulum
Which plasma membrane proteins allow calcium to enter the cell in response to depolarisation?
Dihydropyridine receptors
What happens to calcium once it has passed into the cell via dihydropyridine receptor channels?
It binds to ryanodine receptors on the SR + causes Ca2+ release from the SR
How does calcium stimulate contraction?
It binds to troponin on Actin
What are the different ways in which calcium is removed from the myoplasm after it has stimulated contraction?
Na+/Ca2+ exchanger
SERCA2a (sarcoendoplasmic reticulum calcium ATPase)
What features of contraction is SERCA2a responsible for and why?
Rate of calcium removal: thus rate of cardiac muscle relaxation
Size of calcium store: thus affects contractility of the subsequent beat
What are the 3 main channels that are responsible for the action potential in the sinoatrial node?
If channel: hyperpolarisation-activated cyclic nucleotide-gated (HCN) channel (funny channel)
Ca2+ channel (Transient + Long-lasting)
K+ channel
Describe how channels are responsible for the action potential of the sinoatrial node.
If channels open in hyperpolarised state, utilising cAMP, drive in Na+ to initiate depolarisation
Current starts to rise, stimulating the opening of Ca2+ channels (T then L), which further depolarises the membrane.
K+ channels are responsible for repolarisation
What are beta adrenoceptors coupled with?
Adenylate cyclase: it increases cAMP, which is important in the opening of the If channel to begin depolarisation
How does the parasympathetic nervous system affect heart rate and contractility?
It is negatively coupled with adenylate cyclase, thus decreases cAMP + interferes with funny currents, slows HR
Promotes K+ channel opening
What are the determinants of myocardial oxygen supply?
Arterial oxygen content
Coronary blood flow
What are the determinants of myocardial oxygen demand (work)?
Heart rate
Contractility
Preload
Afterload
What effect do beta-blockers and calcium channel blockers have on the channels responsible for the SA node action potential?
B-blockers decrease If + Ca2+ channel activity
Ca2+ channel blockers decrease Ca2+ channel activity
Thus both decrease HR + contractility
Name a drug that decreases If activity.
Ivabradine (blocks the If channel)
What effect does Ivabradine have on contractility?
No effect on contractility because it doesn’t affect the Ca2+ channels
What are the 2 types of calcium channel blocker? Name the drugs in each category including their drug class.
Rate slowing Phenylalkylamines– Verapamil Benzothiazepines– Diltiazem Non-rate slowing Dihydropyridines– Amlodipine
What is a consequence of non-rate slowing calcium channel blockers?
Reflex tachycardia due to profound vasodilation
How do organic nitrates cause vasodilation?
Organic nitrates are substrates for NO production
NO diffuses into smooth muscle + causes relaxation by activating guanylate cyclase that produces cGMP
cGMP also causes opening of K+ channels + K+ efflux, resulting in hyperpolarisation
How do potassium channel openers work?
They open K+ channels + hyperpolarise the smooth muscle so it is less likely to contract