Dr. Austin New Flashcards
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Classification of Rabies virus
•Rabies is a Lyssavirus that has a linear single stranded negative sense RNA.
•Particle is:
o Enveloped
o Helical
•Replicates in the cytoplasm of host neuronal cells; very little destruction
•Persistent virus that disrupts neuronal function; NOT cytocidal
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b. Serotypes of vesicular stomatitis virus (named after states)
•New jersey and Indiana (but never found in these states)
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c. Match up viruses to their diseases, characteristics, and features
- Rhabdoviridae- Lyssaviruses,(Rabies)-liner, single stranded RNA of negative sense; replicates in cytoplasm
- Vesiculoviruses (Vesicular Stomatitis Virus)-horses, cows, pigs, deer, and humans
- unclassified viruses (Bovine Ephemeral Fever)
- Mokola-Lyssavirus-Nigerian shrews, CNS
- Spring viremia-abdomen dropsy of carp
- Hemorrhagic septicemia virus-Unclassified-European salmonids
- Infectious Hematopoietic Necrosis Virus-Salmonids
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Number of proteins rabies virus codes for
5
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d. Know about street rabies versus sylvatic rabies vs fixed rabies (we do not have street rabies in US because of vaccination program)
•Sylvatic= non domesticated animals, wildlife, Raccoons (FL GA NE states) bats(pan-continental) Foxes, skunks, raccoons, bats, coyotes
-Street=domesticated animals (dog and cat) eradicated in USA
•Fixed rabies viruses= cell, egg, & lab animal cultures (attenuated)
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Where is it endemic and where is it not?
Look at the map in the notes
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f. Know the gross pathogenesis of rabies
- Virus inoculated and uncoated in the myocytes (replication is limited here)
- Transmission to nerve cell at NM junction and sensory endings of PNS
- Centripetal migration up the PNS to DRG
- Enters spinal cord and ascent to brain via cell-cell is fast
- Localizes in soma of neurons in hippocampus, brain stem and purkinje cells (negri bodies)
- Centrifugal descent down PNS to retina, cornea, salivary glands, nasal mucosa, hair and taste buds.
- Virus released at nerve endings
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g. Where does transmission of rabies occur on neuron (the receptor it uses to get into PNS)
•At the neuromuscular junction at sensory ending in the PNS (ACH receptor?)
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h. Routes to the CNS information (know the rabies route to the CNS)
- Hematogenous- via viremia, can infect endothelium and leukocytes. Passive transport through permeable areas can occur. Choroid plexus- passage through and replication in the ependymal cells
- Neural- axons, schwann cells, perineural lymphatics and endoneurial spaces
- Olfactory- cranial nerve penetrates cribriform plate, rare for rabies
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Genomic classification to the rabies virus (genome type is has)
- Lyssavirus that has a linear single-stranded RNA of negative sense
- Genome is ~12 Kilobases in size and codes for 5 proteins
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Know about negri bodies (composed of what, location –> in cell and brain)
IC inclusions in neuronal soma present in ganglion of hippocampus, purkinje cells of cerebellum and medulla.
They are composed of ribonuclear proteins
Know how to morphologically characterize the calicivirus
•Virions have a simple construction and consists of a capsid
oCapsids appear round to hexagonal in outline with 32 cup shaped depressions
ocapsid/nucleocapsid= round with icosahedral symmetry
•NOT enveloped
Genomic characteristics of calici
SS RNA positive sense linear naked infectivity
Know about San Miguel seal lion virus and what it is synonymous with
- Vesicular, neonatal hemorrhagic syndrome of sea lions and pinnipeds
- Synonymous with vesicular exanthema of swine
Lists of groups of viruses and diseases –> List out all of the hemorrhagic viruses (comprehensive too)
•African Swine fever •Classical Swine Fever •Canine herpesvirus •Chicken anemia virus •San Miguel sea lion virus •Vesicular exanthema of swine viruses •Feline calicivirus •Lagovirus- rabbit calicivirus disease •Ranavirus Red Disease of Pike •Pseudorabies is NOT
