DNA transcription - Exam Questions

Question 1

Which polynucleotides read in the 5’ to 3’ direction?

Answer 1

The DNA coding strand and mRNA

Question 2

Initiation of transcription in prokaryotes requires..:

a. RNA polymerase to bind to the Pribnow box
b. Sigma factor to dissociate from the Pribnow box
c. Sigma factor to bind to the Pribnow box and then RNA polymerase to bind
d. the Pribnow box to bind to the transcription start site, then RNA polymerase to bind
e. Sigma factor to bind to the transcription start site, then RNA polymerase to displace it

Answer 2

c. Initiation of transcription in prokaryotes requires the sigma factor to bind to the Pribnow box and then RNA polymerase to bind

Question 3

mRNA detachment from DNA occurs due to a repeated mRNA sequence of…?

Answer 3

U

Question 4

Introns are regions of DNA that….

Answer 4

….are transcribed but not translated

Question 5

mRNA is capped by joining…

Answer 5

7-methylguanosine to the 5’end via a 5’-5’ triphosphate bridge

Question 6

The end sections of mRNA contains….

Answer 6

….50-250 adenosine residues added to the 3’ end

Question 7

hnRNA is mRNA…

Answer 7

…which has not yet undergone splicing