DNA Structures Flashcards
Purines
N
Pyrimidines
Nitrogenous bases with one ring: Cytosine (C) and Thymine (T)
Base Pairing Rules
A-T with 2 hydrogen bonds, C-G with 3 hydrogen bonds
Antiparallel
The two strands of DNA run in opposite directions (5’→3’ and 3’→5’)
B-DNA
The most common right-handed double helix form of DNA
DNA Polymerase III
Adds nucleotides to the new DNA strand in the 5’→3’ direction
Helicase
Unwinds and separates the DNA strands
SSBs
Single-strand binding proteins that stabilize open DNA strands
Primase
Adds RNA primers to initiate DNA synthesis
Ligase
Seals gaps between Okazaki fragments on the lagging strand
Leading Strand
Synthesized continuously in the 5’→3’ direction
Lagging Strand
Synthesized in short segments called Okazaki fragments
Semiconservative Replication
Each new DNA molecule has one old strand and one new strand
Phosphodiester Bond
Link between the 3’ OH and 5’ phosphate of nucleotides
Direction of Synthesis
DNA is synthesized in the 5’→3’ direction