DNA structure & replication Flashcards
CH. 14
central dogma
DNA → RNA → protein
(replication) transcription → translation
Frederick Griffith
Proved there is hereditary element
- pre-biotic era
- used mice to study S. pneumoniae
- shiny/smooth strain = deadly
- rough strain = killable
Griffith experiment
- rough strain → mouse lives
- smooth strain → mouse dies
- heat-killed smooth strain → mouse lives
- rough strain + heat-killed smooth strain → mouse dies
Heat-killed smooth strain mixed with rough strain caused rough strain to mutate into smooth strain
Avery, Macleod & McCarty
showed DNA is hereditary factor mentioned in Griffith experiment
- developed transformation procedure using extract from heat killed smooth strain
Avery, MacLeod & McCarty experiment
proved DNA is hereditary element in Griffith experiment
- treated heat-killed smooth bacteria with 3 enzymes: protease, DNase & RNase
- result: DNase → mouse lives
- DNA destroyed by DNase
protease
enzyme that catalyzes proteins
DNase
enzyme that catalyzed DNA
RNase
catalytic enzyme for RNA
Hershey-Chase experiment
- T2 bacteriophage
- infection begins by phage attaching to bacteria cell surface
- new phase made inside bacterial cell
- T2 have DNA + protein (no RNA)
- DNA labeled with 32P
- proteins labed with 35P
- all blended
- removes ghosts
- should find 32P labels in bacterial cells if DNA = hereditary material
what is DNA made up of?
phosphates
what is RNA made up of?
sulfur
DNA structure
- nucleotide
- sugar
- nitrogenous bases
nucleotide components
- sugar
- nitrogenous base
- phosphate
sugar components
1’ - base
2’ - OH or H
3’ - OH
4’ - 5’
5’ - phosphate
name types of nitrogenous bases
- pyrimidine - single ring
- thymine
- cytosine
- uracil (RNA)
- “Cut the Py”
- purine - double ring
- adenine
- guanine
- “Pure things Are Good”
Rosalind Franklin
used x-ray diffraction to examine crystal strucure of DNA
- her research used by Watson & Crick to determine DNA structure
- uncredited
- key findings
- duplex
- constant diameter
- phosphates on outside
- 10 bases per turn
Edwin Chargaff
found %T = %A and %G = %C
- Watson & Crick used his research to figure out DNA structure
DNA characteristics
- antiparallel strands
- 5’ to 3’ end
- A-T and G-C
- phosphodiester & hydrogen bonds
why are G-C bonds stronger than A-T bonds?
G-C bonds have 3 H-bonds while A-T bonds have 2 H-bond
phosophodiester bonds
covalent bonds btwn 2 nucleotides in nucleic acid strain
- btwn 5-phosphate & 3-OH of adjacent nucleotide
pyrimidine
single ring
- thymine
- cytosine
- uracil (RNA)
Cut the Pi – pie = 1 circle