DNA Structure and replication Flashcards
Prokaryote, Eukaryote chromosome, and Plasmids
prokaryotes- are haploid (single chromosome). prokaryote contain plasmids ( small molecules replicate by themself)
Eukaryote - are diploid. ( double copy of chromosome from each parent). fungi, algea, and protozoa contain plasmids
DNA pairing
A-T, G-C
RNA pairing
A-U, G-C
methylation group
it represses the gene transcription ( Stop) if added to DNA
Transcription and Translation in Eukaryotes ( describe)
in the nucleus, Translation RNA takes place (DNA has histones and its linear). RNA leaves nucleus has introns and pre mRNA PROCESSING occurs in Cytoplasm where cut introns and keep exons and put a cap and take place. now TRANSLATION takes place. mRNA attaches to the ribosome for translation. three steps of translation. INITIATION- ribosomes bind to mRNA to start codon. ELONGATION- polypeptide chain elongates by successively adding amino acids. TERMINATION- when stop codon is encountered polypeptide is released
Transcription and translation in prokaryote
TRANSCRIPTION takes place in the cytoplasm. circular DNA. no introns/ no mRNA processing. Translation and Transcription take place at the same time. on the promotor site (RNA polymerase attach) ribosome attaches start codon start and stop codon stop.
operon: operator, Gene,
operator where repressor bind. genes are at the end of operon which attaches to polymerases new enzymes
lactose operon
when no sugar operon is off but as soon available bacteria want operon to be on so enzymes can be made and use the sugar to make ATP.
lac operon attaches to the repressor and changes its shape. so the repressor can move away from operon.
Trp operon
controls the production of tryptophan an anmino acid.
bacteria produce continuously. so when too much in the environment, it activates the repressor which attaches to the repressor and turn of the operon. also called repressor operon.
Regulatory genes
regulatory are often expression of the proteins. it typically codes for a repressor which bind to the promoter or operator
Framshift mutation (deletion or insertion)
major difference in Amino acid sequence
Silent mutation
only one change but no change in amino acid sequence
Missence (one base change)
slightly different amino acid sequence
Transformation
when cell takes up DNA from enviornment
Transduction
virus effects it. virus transfer genes into a prokaryote