DNA structural and functional Flashcards
A phosphodiester bond joins
two nuleotides forming a dinucleotide
sequences are always written in which direction?
5’ -3’
The major groove always has - proteins
4 - H bond acceptor, H bond donor, H atoms, Methyl group
the minor groove always has - proteins
3
the twist angle/rotation per residue is
28 - 42
how many base pairs are there during one 360 turn
10.5
Properties of B DNA (diameter, A/bp, bp/turn)
- 5 A
- 4 A/bp
- 4 bp/turn
Properties of A DNA (diameter, A/bp, bp/turn)
25.5 A
2.3 A/bp
11 bp/turn
bases are tilted to 19’
anthrax is due to which bacteria
bacillus antracis
what produces neurotoxin botulinum
clostridium botulinum: the botulinum toxin causes severe flaccid paralytic disease. Are destroyed at 100’. Produces heat resistant spores
Botulinum toxin is also used for?
paralytic effects of human voluntary musculature.
Z and B DNA are both which sided?
left handed
what is the repetitive unit in Z-DNA
a dinucleotide
is Z DNA more stable than B DNA
no, due to the electrostatic repulsion between the negatively charged phosphates on opposite strands which are nearer in this conformation
what is the function of Z-DNA
it can relax DNA supercoils that are produced when the 2 strands are separated thus facilitating gene expression
