DNA replication to chromosome Flashcards

1
Q

Watson and Crick

A

features of DNA helix

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2
Q

James Watson

A

double helix

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3
Q

Meselson and Stahl

A

semi-conservative model

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4
Q

John Cairns

A

replication begins at oriC in E. Coli

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5
Q

what do you need for DNA replication in prokaryotes

A
primer with free OH group at 3' end
template DNA
dNTPs
Mg
DNA polymerase
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6
Q

Polymerase l, lll

A

chromosomal replication

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7
Q

polymerase ll, lV, V

A

DNA repair

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8
Q

pol l

A

removal of RNA primer and filling gaps

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9
Q

pol lll

A

main replicative pol.

Okazaki

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10
Q

DNA ligase

A

closes the nicks in DNA in lagging strand

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11
Q

Robert lehman

A

ligase

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12
Q

dnaA

A

initiator proteins

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13
Q

dnaB

A

helicase

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14
Q

dnaC

A

loader of helicase

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15
Q

dnaG

A

primase produces RNA primer

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16
Q

primers

A

used to iniate DNA synthesis

17
Q

helicase

A

unwinds

18
Q

SSB

A

keeps DNA straight

19
Q

topoisomerase

A

release torsional stress

20
Q

primosome

A

responsible for primer synthesis

21
Q

repliosome

A

responsible for replication at each fork

22
Q

telomeres

A

protects chromosome ends from being degraded