DNA replication Prokaryotes

Question 1

First protein to bind to Oric?

Answer 1

DnaA

Question 2

Where does DnaA bind to?

Answer 2

9bp repeats on Oric

Question 3

What is induced as a result of DnaA binding?

Answer 3

Unwinding at 13bp repeats

Question 4

What is primase?

Answer 4

RNA polymerase
Self-priming
Add RNA 5’-3’ (GTA strand preferred)
No proofreading activity

Question 5

The activity of primase is increased by which enzyme?

Answer 5

Helicase
Cooperatively

Question 6

How do we prevent DNA from closing up again?

Answer 6

Single-stranded binding protein
Binds ssDNA
Prevents reannealing

Question 7

The main replicative enzyme?

Answer 7

Pol III holoenzyme

Question 8

What recruits the arrival of Pol III holoenzyme?

Answer 8

3’ end
Primer +SSB

Question 9

Structure of Pol III holoenzyme?

Answer 9

core - alpha, e, theta (3 subunits)
3 Tau proteins- flexible
Clamp loader
Assessory proteins delta delta, xY (binds SSB)

Question 10

Function of clamp loader?

Answer 10

Loads Beta Clamp onto DNA
Pol III core binds to clamp = starting point
3’

Question 11

The loading strand has what attached?

Answer 11

Polymerase Core

Question 12

How loader detach?

Answer 12

ATP hydrolysis

Question 13

What gene encodes beta clamp?

Answer 13

DnaN