DNA Replication Flashcards
What does the primer do in DNA replication?
It is a short fragment of ss nucleic acid bound to the template which provides a 3’ hydroxyl group to make the next addition.
What is a dNTP and what is a dNMP?
dNTP are building blocks that we add on, the dNMPs are incorporated onto the 3’ end of the primer after it is laid down onto the template strand.
Explain the way in which dNTPs form a strand.
The dNTP is base-paired to the template and is then joined to the backbone by the polymerase. There is a reaction between the 5; phosphate of the dNTP and the 3’ of the OH of the existing chain. (Physical card 8).
What is the initiation site for replication in E. Coli?
OriC.
What is the origin site recognized by in replication? What happens after it is recognised.
An initiator protein DNAA. 20-30 DNAA monomers bind to origin and they recruit helicase to start unwinding.
Refer to physical cards 9 and 10 for an explanation of replication in the leading and lagging strand.
9 and 10.
Where is the primer laid down and by what?
At the origin, the primer is laid down by a primase enzyme.
