DNA replication

Question 1

Step 1

Answer 1

Helicase uncoils the DNA

Question 2

Step 2

Answer 2

RNA primase adds short sequences of RNA to both strands (primer)

Question 3

Step 3

Answer 3

The primer allows the DNA polymerase III to bind and start replication

Question 4

Step 4

Answer 4

DNA polymerase III adds nucleotides to each template strand in a 5’ - 3’ direction

Question 5

Step 5

Answer 5

These nucleotides are intially dioxyribonucleoside triphosphates but they lose two phosphates during the replication to release energy

Question 6

Step 6

Answer 6

One strand is replicated in a continuous manner in the same direction as the replication fork (lagging strand)

Question 7

Step 7

Answer 7

The other strand is replicated in Okazaki fragments in the opposite direction (lagging strand)

Question 8

Step 8

Answer 8

DNA polymerase I removes the RNA primers and replaces them with DNA

Question 9

Step 9

Answer 9

DNA ligase then joins the Okazaki fragments together to form a continuous strand