DNA Replication Flashcards
DNA replication
Each strand acts as a template
Bidirectional Replication
Replication forks move in opposite directions
Origins of Replication
In eukaryotes Replication begins at many different sites
Nothing happens
On its own; many proteins and enzymes
Antiparallel
One moves up; the other down
Unidirectional orientation
New nucleotides can only be added to the 3’ end
Polymerase
Enzymes that synthesize DNA
Can only extend in the 5-3 direction
Primers
Required to begin synthesis
Primase
Creates primers as a type of RNA polymerase
Binding Protein
Binds strands back together
Topisomase
Uncoils/flattens DNA for Helicase to unzip
Helicase
Unwinds genes
Leading strand
Follows moving fork
Lagging strand
Runs away from replication fork
Okazaki Fragments
Short pieces of DNA
Lagging strand