DNA Replication

Question 1

Replication structure

Answer 1

Nucleotide sequence of a DNA strand is copied into a complimentary DNA sequence.
Requires complimentary base pairing.

Question 2

Replication process

Answer 2

DNA polymerisation is catalysed by specific enzymes.
DNA polymerase, addition of a deoxyribonucleotide to the 3’ end of a polynuc chain.
Okazaki fragments joined later by a different enzyme.
Lagging strand replication is done in chunks rather than continuously in the leading strand.

Question 3

T melting

Answer 3

Temperature at which DNA denatures, and the strands separate.
DNA with more C and G has a higher T melting as they have 3 H bonds, A and T have 2 H bonds.

Question 4

Replication fork

Answer 4

Replication occurs when the double helix forms a Y-shaped structure.
Spinning out two daughter DNA helices behind.

Question 5

Asymmetrical pattern

Answer 5

Leading strand grows continuously
Lagging strand is synthesized by a DNA polymerase through backstitching.