DNA Replication

Question 1

Where does DNA Replication take place?

Answer 1

Nucleus

Question 2

When does DNA replication take place?

Answer 2

During S phase (cell cycle)

Question 3

What is the role of helicase?

Answer 3

Unwinds DNA double helix at the replication fork

Question 4

What is single stranded binding proteins role?

Answer 4

Prevents separated strands of DNA from coming back together

Question 5

Is single stranded binding proteins considered an enzyme?

Answer 5

No

Question 6

What is the role of topoisomerase/DNA gyrase?

Answer 6

Relaxes DNA double helix from the tension and supercoiling

Question 7

What is the role of primase?

Answer 7

Places the RNA primers at the origin of replication, initiating DNA replication

Question 8

What is the role of DNA polymerase III?

Answer 8

Builds new DNA strand by adding nucleotides in 5->3 direction

Question 9

What is the role of DNA polymerase I?

Answer 9

Removes RNA primer laid by primate & adds complementary DNA

Question 10

What is the role of DNA Ligase?

Answer 10

Re-anneals semi-conservative strands & joins Okazaki fragments on the lagging strand

Question 11

What is the role of telomerase?

Answer 11

-Synthesize telomeres
-Ensures the chromosomes do not shorten
-Use their own RNA primers